Human FGFR3 (IIIb) Antibody
R&D Systems | Catalog # MAB1474
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Neutralization
Cited:
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 136315
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Product Specifications
Immunogen
Pool of NS0-derived recombinant human FGF R3 alpha (IIIb) and Sf21-derived FGF R3 alpha (IIIc)
Specificity
Detects human
FGF R3 (IIIb) in direct ELISAs. In direct ELISAs, approximately 25‑50%
cross-reactivity with recombinant mouse FGF R3 (IIIb) is
observed and 12% with recombinant human (rh) FGF R4 is
observed and no cross-reactivity with (IIIc) isoforms of rhFGF R3 or any
isoforms of rhFGF R1 or rhFGF R2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human FGFR3 (IIIb) Antibody
FGF R3 Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Human FGF R3 Antibody.
Recombinant Human FGF R3 (IIIb) Fc Chimera (Catalog # 1264-FR) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Human FGF R3 (IIIb) Fc Chimera (300 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human FGF R3 (IIIb) Monoclonal Antibody (Catalog # MAB1474). The ND50 is typically 0.5-2 µg/mL in the presence of heparin (10 µg/mL).Applications for Human FGFR3 (IIIb) Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize FGF R3-mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 300 ng/mL Recombinant Human FGF R3 (IIIb) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FGFR3
Long Name
Fibroblast Growth Factor Receptor 3
Alternate Names
CD333, CEK, FGF R3, JTK4
Gene Symbol
FGFR3
Additional FGFR3 Products
Product Documents for Human FGFR3 (IIIb) Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FGFR3 (IIIb) Antibody
For research use only
Related Research Areas
Citations for Human FGFR3 (IIIb) Antibody
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