Human Flt‑3 Ligand Biotinylated Antibody (Catalog # BAF308)
Recombinant Human Flt-3 Ligand Protein (Catalog # 308-FKN)
Measured by its ability to neutralize Flt‑3 Ligand-induced proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 1 ng/mL Recombinant Human Flt‑3 Ligand.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by Flt‑3 Ligand and Neutralization by Human Flt‑3 Ligand Antibody. Recombinant Human Flt‑3 Ligand (Catalog # 308-FK) stimulates proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt‑3 Ligand (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt‑3 Ligand Monoclonal Antibody (Catalog # MAB608). The ND50 is typically 0.02‑0.06 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Flt-3 Ligand
Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.
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