Human Flt-3 Ligand/FLT3L Antibody

Catalog # Availability Size / Price Qty
MAB608-SP
MAB608-500
MAB608-100
Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody.
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Product Details
Citations (2)
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Human Flt-3 Ligand/FLT3L Antibody Summary

Species Reactivity
Human
Specificity
Detects human Flt-3 Ligand/FLT3L in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 40416
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Flt-3 Ligand/FLT3L
Thr27-Pro185
Accession # AAA17999
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Flt‑3 Ligand/FLT3L (Catalog # 308-FK)

Human Flt-3 Ligand/FLT3L Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Flt-3 Ligand/FLT3L Biotinylated Antibody (Catalog # BAF308)

Standard: Recombinant Human Flt-3 Ligand/FLT3L Protein (Catalog # 308-FKN)

Neutralization
Measured by its ability to neutralize Flt‑3 Ligand/FLT3L-induced proliferation in BaF3 mouse pro‑B cell line transfected with mouse Flt‑3. Hannum, C. et al. (1994) Nature 368:643. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 1 ng/mL Recombinant Human Flt‑3 Ligand/FLT3L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody. View Larger

Cell Proliferation Induced by Flt-3 Ligand/FLT3L and Neutralization by Human Flt-3 Ligand/FLT3L Antibody. Recombinant Human Flt-3 Ligand/FLT3L (Catalog # 308-FK) stimulates proliferation in BaF3 mouse pro-B cell line transfected with mouse Flt-3 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Flt-3 Ligand/FLT3L (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Flt-3 Ligand/FLT3L Monoclonal Antibody (Catalog # MAB608). The ND50 is typically 0.02-0.06 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as Flk-2 Ligand, is a transmembrane protein that can be cleaved to release a soluble cytokine. Flt-3 Ligand binds Flt-3 and promotes the growth and differentiation of early hematopoietic progenitor cells.

Long Name
fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs
2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names
FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine

Product Datasheets

Citations for Human Flt-3 Ligand/FLT3L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.
    Authors: Anandasabapathy N, Breton G, Hurley A, Caskey M, Trumpfheller C, Sarma P, Pring J, Pack M, Buckley N, Matei I, Lyden D, Green J, Hawthorne T, Marsh H, Yellin M, Davis T, Keler T, Schlesinger S
    Bone Marrow Transplant, 2015;50(7):924-30.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  2. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002;168(9):4326-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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