|Detection of Human FoxC1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, NCI‑H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human FoxC1 Monoclonal Antibody (Catalog # MAB6329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.|
Human FoxC1 (forkhead box C1), also called FKHL7 (forkhead-like 7) or FREAC3 (forkhead-related activator 3) is a 553 amino acid (aa) intracellular phosphoprotein that belongs to a large family of nuclear transcription factors that share a common forkhead/winged helix DNA binding domain. FoxC1 is essential for formation of mesodermal tissues; mutations underlie Axenfeld-Rieger malformations of the eye. The human FoxC1 sequence includes a DNA binding domain at aa 77‑168 and an inhibitory domain between aa 268‑245. Phosphorylation within this inhibitory region causes a protein gel mobility shift. Human FoxC1 shares 92% and 91% aa identity with mouse and rat FoxC1, respectively.
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