Human FPRP/PTGFRN Antibody

Catalog #: MAB100431 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB100431-100
MAB100431-SP
Detection of FPRP/PTGFRN in U87-MG Human Cell Line by Flow Cytometry
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Human FPRP/PTGFRN Antibody Summary

Species Reactivity
Human
Specificity
Detects human FPRP/PTGFRN in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 998107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived human FPRP/PTGFRN
Pro27-Pro832
Accession # Q9P2B2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FPRP/PTGFRN antibody in U87-MG Human Cell Line antibody by Flow Cytometry View Larger

Detection of FPRP/PTGFRN in U87-MG Human Cell Line by Flow Cytometry U87-MG human glioblastoma cell line was stained with Mouse Anti-Human FPRP/PTGFRN Monoclonal Antibody (Catalog # MAB100431, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FPRP/PTGFRN

FPRP (F2 alpha Prostaglandin receptor Regulatory Protein; also CD315, EWI-F and CD9 partner-1/P-1) is a 130 kDa type I transmembrane (TM) glycoprotein member of the EWI subfamily, Ig superfamily of molecules. It associates with the 7-TM prostaglandin F2 alpha  receptor, inhibiting its activity. It also forms complexes with 4-TM CD9 and CD81, linking these molecules to the intracellular actin cytoskeleton.

Long Name
Prostaglandin F2-alpha Receptor Regulatory Protein
Entrez Gene IDs
5738 (Human); 19221 (Mouse); 29602 (Rat)
Alternate Names
CD315; CD9P-1; FPRP; PTGFRN

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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