Human G-CSFR/CD114 Antibody (38643)

R&D Systems | Catalog # MAB3811

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 38643
View all G-CSFR/CD114 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human G-CSF R/CD114
Glu25-Pro621
Accession # Q99062

Specificity

Detects human G-CSF R/CD114 in ELISAs and Western blots. In sandwich Immunoassays, no cross-reactivity with recombinant human (rh) G‑CSF, rhGM‑CSF R beta, rhGM‑CSF R, or recombinant mouse G‑CSF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human G-CSFR/CD114 Antibody (38643)

Detection of Human G-CSF R/CD114 antibody by Western Blot.

Detection of Human G‑CSF R/CD114 by Western Blot.

Western blot shows lysates of AML-193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human G-CSF R/CD114 Monoclonal Antibody (Catalog # MAB3811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for G-CSF R/ CD114 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human G-CSFR/CD114 Antibody (38643)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human whole blood monocytes and human whole blood granulocytes

Western Blot

0.5 µg/mL
Sample: AML‑193 human acute monocytic leukemia cell line

Human G-CSF R/CD114 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human G-CSF R/CD114 Biotinylated Antibody (Catalog # BAF381)
  • Standard: Recombinant Human G-CSFR/CD114 Protein (Catalog # 381-GR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using MAB3811 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: G-CSFR/CD114

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant. Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 863 amino acids (aa) in length, with a 604 aa extracellular domain, a 26 aa transmembrane domain, and a 183 aa cytoplasmic domain that include a 23 amino acid signal sequence. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor; however the existence of soluble G-CSF R in human serum has not been reported (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (2). Human and mouse G-CSF R have a homology of 62.5%. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1).

References

  1. Nicola, N.A., in Cytokine Reference, (2001) Oppenhiem, J.J. and M. Feldmann, eds. Academic Press p. 1935
  2. Mitsui, T. et al. (2003) Blood. 101:2990.

Long Name

Granulocyte Colony Stimulating Factor Receptor

Alternate Names

CD114, CSF3R, Csfgr, G-CSF R, GCSFR

Entrez Gene IDs

1441 (Human); 12986 (Mouse)

Gene Symbol

CSF3R

UniProt

Q99062 (Human)

Additional G-CSFR/CD114 Products

Product Documents for Human G-CSFR/CD114 Antibody (38643)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human G-CSFR/CD114 Antibody (38643)

For research use only

Citations for Human G-CSFR/CD114 Antibody (38643)

Customer Reviews for Human G-CSFR/CD114 Antibody (38643) (2)

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Showing  1 - 2 of 2 reviews Showing All
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  • Human G-CSFR/CD114 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/13/2019
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human G-CSFR/CD114 Antibody (38643) MAB3811
  • Human G-CSF R/CD114 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/11/2018

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