Human Galectin-7 Antibody

(3 citations)
(4 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse (rm) Galectin-7 is observed and less than 0.5% cross-reactivity with recombinant human (rh) Galectin-1, rmGalectin-3, rhGalectin-4, and rhGalectin-8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Galectin‑7
    Ser2-Phe136
    Accession # NP_002298
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human skin
    • Human Galectin-7 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human Galectin‑7 Antibody (Catalog # AF1339)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Galectin‑7 Biotinylated Antibody (Catalog # BAF1339)
  • ELISA Standard
     
    Recombinant Human Galectin-7 Protein (Catalog # 1339-GA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Galectin‑7 by Simple WesternTM. Simple Western lane view shows lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Detection of Human Galectin‑7 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin‑7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Human Galectin-7 is a prototype monomeric galectin. It is specifically expressed in stratified epithelia, notably in epidermis, but is barely detectable in epidermal tumors and significantly down regulated or absent from squamous carconima cell lines. The Galectin-7 gene is induced by tumor suppressor protein p53 transcriptional activity following genotoxic events. A pro-apoptotic protein, Galectin-7 functions intracellularly upstream of JNK activation and cytochrome-c release. This protein has been shown to increase the susceptibility of keratinocytes to UVB induced apoptosis, an essential processss in the maintenance of epidermal homeostasis. Cell lines transfected with the Galectin-7 gene localized the protein in the nucleus and intracellularly. Human and mouse Galectin-7 share 79% amino acid homology (4-6).

  • References:
    1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
    2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
    3. Hughes, R.C. (2002) Biochimie 83:667.
    4. R&D Systems Cytokine Bulletin; Summer 2002.
    5. Bernerd, F. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11329.
    6. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.
  • Entrez Gene IDs:
    3963 (Human); 16858 (Mouse)
  • Alternate Names:
    GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Sample Type
  1. A Mutation in the Carbohydrate Recognition Domain Drives a Phenotypic Switch in the Role of Galectin-7 in Prostate Cancer.
    Authors: Labrie M, Vladoiu M, Leclerc B, Grosset A, Gaboury L, Stagg J, St-Pierre Y
    PLoS ONE, 2015;10(7):e0131307.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  2. Galectin-7 acts as an adhesion molecule during implantation and increased expression is associated with miscarriage.
    Authors: Menkhorst E, Gamage T, Cuman C, Kaitu'u-Lino T, Tong S, Dimitriadis E
    Placenta, 2014;35(3):195-201.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  3. The CCAAT/enhancer-binding protein beta-2 isoform (CEBPbeta-2) upregulates galectin-7 expression in human breast cancer cells.
    Authors: Campion C, Labrie M, Grosset A, St-Pierre Y
    PLoS ONE, 2014;9(5):e95087.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Average Rating: 4.3 (Based on 4 reviews)

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We have 3 reviews tested in 1 species: Human.
We have 4 reviews tested in 2 applications: Western Blot, Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human Galectin‑7 Antibody AF1339
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Very Good
 WB Anonymous 10/26/2015
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Western Blot Human Galectin‑7 Antibody AF1339
Western Blot: Human Galectin‑7 Antibody [AF1339]

Summary

ApplicationWestern Blot
Sample TestedCancer cell lysates

Other Experimental Details

Other Experimental DetailsFirst run with 1:1000 for difference cancer cell lysates gave many bands, which could be isoforms detected.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: BSA
Dilution: 1:1000
Western Blot Human Galectin‑7 Antibody AF1339
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Very Good
 WB Human Anonymous 10/26/2015
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Western Blot Human Galectin‑7 Antibody AF1339
Western Blot: Human Galectin‑7 Antibody [AF1339]

Summary

ApplicationWestern Blot
Sample TestedCancer cell lysates
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAt higher dilutions, only the main form was visible.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Loading buffer
Dilution: 1:2000
Immunohistochemistry Human Galectin‑7 Antibody AF1339
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Excellent
 IHC Human Anonymous 10/26/2015
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Immunohistochemistry Human Galectin‑7 Antibody AF1339
Immunohistochemistry: Human Galectin‑7 Antibody [AF1339]

Summary

ApplicationImmunohistochemistry
Sample TestedColon tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAt 1:500 the antibody is very efficient in detecting galectin-7.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:500
Immunohistochemistry Human Galectin‑7 Antibody AF1339
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Very Good
 IHC Human Anonymous 10/26/2015
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Immunohistochemistry Human Galectin‑7 Antibody AF1339
Immunohistochemistry: Human Galectin‑7 Antibody [AF1339]

Summary

ApplicationImmunohistochemistry
Sample TestedColon tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsAt 1:1000 there was low detection levels of Gal-7.
Specificity: Not Specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000

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