Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair), Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Galectin-7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Galectin‑7
Ser2-Phe136
Accession # NP_002298

Specificity

Detects human Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse (rm) Galectin-7 is observed and less than 0.5% cross-reactivity with recombinant human (rh) Galectin-1, rmGalectin-3, rhGalectin-4, and rhGalectin-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Galectin‑7 Antibody

Detection of Human Galectin-7 antibody by Western Blot.

Detection of Human Galectin‑7 by Western Blot.

Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Galectin-7 antibody by Simple WesternTM.

Detection of Human Galectin‑7 by Simple WesternTM.

Simple Western lane view shows lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Galectin‑7 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Simple Western

10 µg/mL
Sample: HEK001 human epidermal keratinocyte cell line

Western Blot

1 µg/mL
Sample: Human skin tissue

Human Galectin-7 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Galectin‑7 Biotinylated Antibody (Catalog # BAF1339)
  • Standard: Recombinant Human Galectin-7 Protein (Catalog # 1339-GA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using AF1339 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Human Galectin-7 is a prototype monomeric galectin. It is specifically expressed in stratified epithelia, notably in epidermis, but is barely detectable in epidermal tumors and significantly down regulated or absent from squamous carconima cell lines. The Galectin-7 gene is induced by tumor suppressor protein p53 transcriptional activity following genotoxic events. A pro-apoptotic protein, Galectin-7 functions intracellularly upstream of JNK activation and cytochrome-c release. This protein has been shown to increase the susceptibility of keratinocytes to UVB induced apoptosis, an essential processss in the maintenance of epidermal homeostasis. Cell lines transfected with the Galectin-7 gene localized the protein in the nucleus and intracellularly. Human and mouse Galectin-7 share 79% amino acid homology (4-6).

References

  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems Cytokine Bulletin; Summer 2002.
  5. Bernerd, F. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11329.
  6. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.

Alternate Names

GAL7, Galectin7, LGALS7

Entrez Gene IDs

3963 (Human); 16858 (Mouse)

Gene Symbol

LGALS7

UniProt

NP_002298

Additional Galectin-7 Products

Product Documents for Human Galectin‑7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Galectin‑7 Antibody

For research use only

Related Research Areas

Lectins
NKG2D Receptors and Ligands

Citations for Human Galectin‑7 Antibody

Customer Reviews for Human Galectin‑7 Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
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4 Stars
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Showing  1 - 4 of 4 reviews Showing All
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  • Human Galectin‑7 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 10/26/2015
    At 1:500 the antibody is very efficient in detecting galectin-7. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS<br />Dilution: 1:500
    Human Galectin‑7 Antibody AF1339
  • Human Galectin‑7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Verified Customer | Posted 10/26/2015
    First run with 1:1000 for difference cancer cell lysates gave many bands, which could be isoforms detected. <br />Specificity: Reasonably specific<br />Sensitivity: Reasonably sensitive<br />Buffer: BSA<br />Dilution: 1:1000
    Human Galectin‑7 Antibody AF1339
  • Human Galectin‑7 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 10/26/2015
    At 1:1000 there was low detection levels of Gal-7. <br />Specificity: Not Specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS<br />Dilution: 1:1000
    Human Galectin‑7 Antibody AF1339
  • Human Galectin‑7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cancer cell lysates
    Species: Human
    Verified Customer | Posted 10/26/2015
    At higher dilutions, only the main form was visible. <br />Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: Loading buffer<br />Dilution: 1:2000
    Human Galectin‑7 Antibody AF1339

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Protocols

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