Human Galectin-7 Antibody

Catalog # Availability Size / Price Qty
AF1339
AF1339-SP
Detection of Human Galectin‑7 by Simple WesternTM.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (4)

Human Galectin-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse (rm) Galectin-7 is observed and less than 0.5% cross-reactivity with recombinant human (rh) Galectin-1, rmGalectin-3, rhGalectin-4, and rhGalectin-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Galectin‑7
Ser2-Phe136
Accession # NP_002298
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin

Human Galectin-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human Galectin‑7 Biotinylated Antibody (Catalog # BAF1339)

Standard: Recombinant Human Galectin-7 Protein (Catalog # 1339-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Simple Western Detection of Human Galectin‑7 by Simple WesternTM. View Larger

Detection of Human Galectin‑7 by Simple WesternTM. Simple Western lane view shows lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Western Blot Detection  of Human Galectin‑7 by Western Blot. View Larger

Detection of Human Galectin‑7 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin‑7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1-4). Human Galectin-7 is a prototype monomeric galectin. It is specifically expressed in stratified epithelia, notably in epidermis, but is barely detectable in epidermal tumors and significantly down regulated or absent from squamous carconima cell lines. The Galectin-7 gene is induced by tumor suppressor protein p53 transcriptional activity following genotoxic events. A pro-apoptotic protein, Galectin-7 functions intracellularly upstream of JNK activation and cytochrome-c release. This protein has been shown to increase the susceptibility of keratinocytes to UVB induced apoptosis, an essential processss in the maintenance of epidermal homeostasis. Cell lines transfected with the Galectin-7 gene localized the protein in the nucleus and intracellularly. Human and mouse Galectin-7 share 79% amino acid homology (4-6).

References
  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems Cytokine Bulletin; Summer 2002.
  5. Bernerd, F. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11329.
  6. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.
Entrez Gene IDs
3963 (Human); 16858 (Mouse)
Alternate Names
GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1

Product Datasheets

Citations for Human Galectin-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers
    Authors: M Labrie, LOF De Araujo, L Communal, AM Mes-Masson, Y St-Pierre
    Sci Rep, 2017;7(1):13244.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. A Mutation in the Carbohydrate Recognition Domain Drives a Phenotypic Switch in the Role of Galectin-7 in Prostate Cancer.
    Authors: Labrie M, Vladoiu M, Leclerc B, Grosset A, Gaboury L, Stagg J, St-Pierre Y
    PLoS ONE, 2015;10(7):e0131307.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC - Paraffin embedded
  3. The CCAAT/enhancer-binding protein beta-2 isoform (CEBPbeta-2) upregulates galectin-7 expression in human breast cancer cells.
    Authors: Campion C, Labrie M, Grosset A, St-Pierre Y
    PLoS ONE, 2014;9(5):e95087.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Galectin-7 acts as an adhesion molecule during implantation and increased expression is associated with miscarriage.
    Authors: Menkhorst E, Gamage T, Cuman C, Kaitu'u-Lino T, Tong S, Dimitriadis E
    Placenta, 2014;35(3):195-201.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Human Galectin-7 Antibody

Average Rating: 4.3 (Based on 4 Reviews)

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Human Galectin‑7 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cancer cell lysates Species: Human

First run with 1:1000 for difference cancer cell lysates gave many bands, which could be isoforms detected.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: BSA
Dilution: 1:1000


Human Galectin‑7 Antibody
By Anonymous on 10/26/2015
Application: WB Sample Tested: Cancer cell lysates Species: Human

At higher dilutions, only the main form was visible.
Specificity: Specific
Sensitivity: Sensitive
Buffer: Loading buffer
Dilution: 1:2000


Human Galectin‑7 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

At 1:500 the antibody is very efficient in detecting galectin-7.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:500


Human Galectin‑7 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Colon tissue Species: Human

At 1:1000 there was low detection levels of Gal-7.
Specificity: Not Specific
Sensitivity: Reasonably sensitive
Buffer: PBS
Dilution: 1:1000