Human Gas6 Antibody

R&D Systems | Catalog # AB885

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Bioassay, ELISA Capture, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Gas6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Gas6
Asp118-Ala678
Accession # NP_000811

Specificity

Detects human Gas6 in ELISAs and Western blots. Because this antibody preparation is a total IgG fraction, complete monospecificity cannot be assumed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human Gas6 Antibody

Detection of Gas6 by Western Blot

Detection of Gas6 by Western Blot

TAMs and CAFs are the main sources of Gas6 in pancreatic tumors. (A) KPCluc/zsGreen (zsGreen) -derived tumor cells (FC1242luc/zsGreen) were orthotopically implanted into the pancreas of syngeneic recipient (C57/BL6) mice. Tumors were harvested and digested at day 23 after implantation and tumor cells, non-immune stromal cells, M1-like and M2-like macrophages were sorted by flow cytometry. Gas6 mRNA levels were quantified in CD45–/zsGreen+ tumor cells, CD45–/zsGreen– non-immune stromal cells, CD45+/F4/80+/CD206– M1-like macrophages and CD45+/F4/80+/CD206+ M2-like macrophages sorted by flow cytometry from murine pancreatic tumors. Values shown are the mean and SD (n = 3). (B) Quantification of Gas6 mRNA expression levels in ex vivo mouse primary isolated macrophages and pancreatic fibroblasts from naïve mice. Values shown are the mean and SD (n = 3). (C) Immunoblotting analysis of Gas6 secreted protein present in mouse macrophage conditioned media (MCM) and pancreatic fibroblast conditioned media (FCM). (D) Images show phospho-Axl, alpha SMA (fibroblast marker) and CD68 (pan-macrophage marker) staining in naïve mouse pancreas and in serial sections of mouse PDA tissues. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32174917), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Gas6 by Immunohistochemistry

Detection of Gas6 by Immunohistochemistry

Gas6 blockade increases NK cell numbers in metastatic lungs and in tumor draining lymph nodes. (A) Immunohistochemical staining of NK cells in metastatic lungs from pancreatic tumor bearing mice treated with control IgG or anti-Gas6 antibody. Lesions indicated by dashed line and NK cells by red asterisk. Scale bar, 50 μm. (B) Quantification of NK cells in metastatic lung tissues from control IgG and anti-Gas6 treated mice. Values shown are the mean and SEM (n = 6 mice in IgG treatment group, n = 7 mice in anti-Gas6 treatment group). **p ≤ 0.01, using unpaired student T-test. (C) Immunofluorescent staining of NK cells in mesenteric lymph nodes from pancreatic tumor bearing mice treated with control IgG or anti-Gas6 antibody. NK marker NKp46 is shown in red, Ki67 is shown in green and nuclei were stained with DAPI (in blue). Scale bar, 50 μm. (D) Quantification of NK cells in tumor draining lymph nodes from control IgG and anti-Gas6 treated mice. Values shown are the mean and SEM (n = 6 mice IgG treatment group and n = 7 mice anti-Gas6 treatment group, 3–6 fields/ mouse tissue were quantified). *p ≤ 0.05, using unpaired student T-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32174917), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Gas6 by Immunohistochemistry

Detection of Gas6 by Immunohistochemistry

Immunohistochemical staining. (A) Representative examples of expression score categories. (B) Distribution of expression scores in the whole HNSCC population and specific subtypes. neg.—negative, we.—weak, mod.—moderate, str.—strong, IHC—immunohistochemistry. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35406601), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Gas6 by Immunohistochemistry

Detection of Gas6 by Immunohistochemistry

Gas6 blockade increases NK cell numbers in metastatic lungs and in tumor draining lymph nodes. (A) Immunohistochemical staining of NK cells in metastatic lungs from pancreatic tumor bearing mice treated with control IgG or anti-Gas6 antibody. Lesions indicated by dashed line and NK cells by red asterisk. Scale bar, 50 μm. (B) Quantification of NK cells in metastatic lung tissues from control IgG and anti-Gas6 treated mice. Values shown are the mean and SEM (n = 6 mice in IgG treatment group, n = 7 mice in anti-Gas6 treatment group). **p ≤ 0.01, using unpaired student T-test. (C) Immunofluorescent staining of NK cells in mesenteric lymph nodes from pancreatic tumor bearing mice treated with control IgG or anti-Gas6 antibody. NK marker NKp46 is shown in red, Ki67 is shown in green and nuclei were stained with DAPI (in blue). Scale bar, 50 μm. (D) Quantification of NK cells in tumor draining lymph nodes from control IgG and anti-Gas6 treated mice. Values shown are the mean and SEM (n = 6 mice IgG treatment group and n = 7 mice anti-Gas6 treatment group, 3–6 fields/ mouse tissue were quantified). *p ≤ 0.05, using unpaired student T-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32174917), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Gas6 Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Human Gas6, lacking the Gla domain, to immobilized Recombinant Human Axl Fc Chimera (Catalog # 154-AL) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.

Western Blot

1 µg/mL
Sample: Recombinant Human Gas6 (Catalog # 885-GS)

Reviewed Applications

Read 2 reviews rated 5 using AB885 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Gas6

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is upregulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF‑like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).

References

  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.

Long Name

Growth-arrest-specific Protein 6

Alternate Names

AXLLG

Entrez Gene IDs

2621 (Human); 14456 (Mouse)

Gene Symbol

GAS6

UniProt

NP_000811

Additional Gas6 Products

Product Documents for Human Gas6 Antibody

Certificate of Analysis

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Product Specific Notices for Human Gas6 Antibody

For research use only

Citations for Human Gas6 Antibody

Customer Reviews for Human Gas6 Antibody (2)

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Customer Images

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  • Human Gas6 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Culture Media
    Species: Human
    Verified Customer | Posted 06/17/2017
  • Human Gas6 Antibody
    Name: Adam Guess
    Application: Flow Cytometry
    Sample Tested: Mesenchymal stem cells
    Species: Human
    Verified Customer | Posted 01/10/2017
    Human Gas6 Antibody AB885

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