Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑4/GDNF R alpha ‑4
Asn24-Ser245 (Ser140Cys) predicted
Accession # NP_071422

Specificity

Detects human GFR alpha ‑4/GDNF R alpha ‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) GFR alpha -1 is observed and less than 1% cross-reactivity with rhGFR alpha -2 and rhGFR alpha -3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human GFR alpha-4/GDNF R alpha-4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human GFR alpha ‑4/GDNF R alpha ‑4

Reviewed Applications

Read 3 reviews rated 4 using AF1439 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-4/GDNF R alpha-4

GFR alpha -4 is a cysteine-rich glycosylphosphotidylinositol GPI-linked cell surface protein that preferentially binds persephin.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 4

Alternate Names

GDNF R alpha-4, GFR alpha4, GFRa-4, GFRA4

Entrez Gene IDs

64096 (Human); 14588 (Mouse)

Gene Symbol

GFRA4

UniProt

Additional GFR alpha-4/GDNF R alpha-4 Products

Product Documents for Human GFR alpha-4/GDNF R alpha-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human GFR alpha-4/GDNF R alpha-4 Antibody

For research use only

Related Research Areas

Customer Reviews for Human GFR alpha-4/GDNF R alpha-4 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
33%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: GFR alpha-4 in transfected HEK293 cells
    Species: Human
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: GFR alpha-4 in transfected HEK293 cells
    Species: Human
    Verified Customer | Posted 12/19/2014
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: GFR alpha-4 in transfected HEK293 cells
    Species: Human
    Verified Customer | Posted 12/19/2014

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FAQs

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