Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑4/GDNF R alpha ‑4
Asn24-Ser245 (Ser140Cys) predicted
Accession # NP_071422
Asn24-Ser245 (Ser140Cys) predicted
Accession # NP_071422
Specificity
Detects human GFR alpha ‑4/GDNF R alpha ‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) GFR alpha -1 is observed and less than 1% cross-reactivity with rhGFR alpha -2 and rhGFR alpha -3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human GFR alpha-4/GDNF R alpha-4 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human GFR alpha ‑4/GDNF R alpha ‑4
Sample: Recombinant Human GFR alpha ‑4/GDNF R alpha ‑4
Reviewed Applications
Read 3 reviews rated 4 using AF1439 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: GFR alpha-4/GDNF R alpha-4
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 4
Alternate Names
GDNF R alpha-4, GFR alpha4, GFRa-4, GFRA4
Gene Symbol
GFRA4
UniProt
Additional GFR alpha-4/GDNF R alpha-4 Products
Product Documents for Human GFR alpha-4/GDNF R alpha-4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human GFR alpha-4/GDNF R alpha-4 Antibody
For research use only
Related Research Areas
Customer Reviews for Human GFR alpha-4/GDNF R alpha-4 Antibody (3)
4 out of 5
3 Customer Ratings
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Application: ImmunoprecipitationSample Tested: GFR alpha-4 in transfected HEK293 cellsSpecies: HumanVerified Customer | Posted 12/19/2014
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Application: ImmunofluorescenceSample Tested: GFR alpha-4 in transfected HEK293 cellsSpecies: HumanVerified Customer | Posted 12/19/2014
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Application: Western BlotSample Tested: GFR alpha-4 in transfected HEK293 cellsSpecies: HumanVerified Customer | Posted 12/19/2014
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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