Human GFR alpha -2/GDNF R alpha -2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GFR alpha ‑2/GDNF R alpha ‑2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 71515
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human GFR alpha ‑2/GDNF R alpha ‑2
    Ser22-Ser441
    Accession # O00451
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GFR alpha ‑2/GDNF R alpha ‑2 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human
GFR alpha ‑2/GDNF R alpha ‑2 Monoclonal Antibody (Catalog # MAB6131, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
GFR alpha ‑2/GDNF R alpha ‑2 in SH‑SY5Y Human Cell Line. GFR alpha ‑2/GDNF R alpha ‑2 was detected in immersion fixed SH‑SY5Y human neuroblastoma cell line using Mouse Anti-Human GFR alpha ‑2/
GDNF R alpha ‑2 Monoclonal Antibody (Catalog # MAB6131) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-2/GDNF R alpha-2

Glial cell line-derived growth factor (GDNF), neurturin (NTN), persephin (PSP) and artemin, distant members of theTGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha -1 [GFR alpha -1], also known as GDNF R alpha -1 or Trn R1, or GFR alpha -2, also known as  GDNF R alpha -2 or Trn R2). GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF or NTN to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase.

Human GFR alpha -2 cDNA encodes a 464 amino acid (aa) residue protein with a putative N-terminal 21 aa hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -2 has a C-terminal hydrophobic region which is preceded by a three aa (GPS) GPI-binding site. Human GFR alpha -2 shares 96.5% amino acid identity with mouse GFR alpha -2. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

  • References:
    1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
    2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
    3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
    4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  • Long Name:
    Glial Cell line-derived Neurotrophic Factor Receptor alpha 2
  • Entrez Gene IDs:
    2675 (Human); 14586 (Mouse)
  • Alternate Names:
    GDNF family receptor alpha 2; GDNF family receptor alpha-2; GDNF R alpha-2; GDNF receptor alpha-2; GDNF receptor beta; GDNFR-alpha-2; GDNFR-beta; GDNFRBRET ligand 2; GFR alpha2; GFR alpha-2; GFRA2; GFRa-2; GFR-alpha 2; GFR-alpha-2; glial cell line derived neurotrophic factor receptor, beta; Neurturin receptor alpha; NRTNR-alpha; NTNRA; NTNR-alpha; PI-linked cell-surface accessory protein; RETL2TGF-beta-related neurotrophic factor receptor 2; TGF-beta related neurotrophic factor receptor 2; TRN receptor, GPI-anchored; TRNR2NRTNR-ALPHA
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