Human Glycine N-methyltransferase/GNMT Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Glycine N-methyltransferase/GNMT in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 691305
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Glycine N-methyltransferase/GNMT
    Met1-Asp295
    Accession # Q14749
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Glycine N-methyltransferase/GNMT by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 1 µg/mL of Human Glycine N-methyltransferase/GNMT Monoclonal Antibody (Catalog # MAB6526) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glycine N-methyltransferase/GNMT at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glycine N-Methyltransferase/GNMT

GNMT (glycine N-methyltransferase) is a cytoplasmic homotetramer of 33 kDa units that converts S-adenosylmethionine to N-methylglycine (sarcosine), also producing S-adenosylhomocysteine. It is abundant in liver, but has also been found in the pancreas and prostate, and is downregulated in hepatocellular carcinoma. GNMT adjusts liver methyl group synthesis for dietary methionine, influencing DNA methylation. The 5-methyltetrahydrofolate form of folic acid binds and inhibits GNMT activity. The 295 amino acid (aa) human GNMT shares 92% aa identity with mouse and rat GNMT.

  • Entrez Gene IDs:
    27232 (Human); 14711 (Mouse); 25134 (Rat)
  • Alternate Names:
    EC 2.1.1.20; Glycine NMethyltransferase; Glycine N-Methyltransferase; GNMT
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