Human Granzyme A Antibody

Catalog #: MAB2905 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2905
MAB2905-SP

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Detection of Human Granzyme A by Western Blot.

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All Granzyme A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Granzyme A Antibody Summary

Species Reactivity

Human

Specificity

Detects human Granzyme A in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Granzyme B, rhGranzyme H, recombinant mouse (rm) Granzyme D, or rmGranzyme G is observed.

Source

Monoclonal Mouse IgG₁ Clone # 356422

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme A
Cys26-Val262
Accession # P12544

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Granzyme A by Western Blot. Western blot shows lysates of NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Granzyme A Monoclonal Antibody (Catalog # MAB2905) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Granzyme A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Granzyme A

Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a 262 amino acid (aa) precursor with a signal peptide (aa 1‑26), a propeptide (aa 27‑28) and a mature chain
(aa 29‑262) (2). After being activated by lysyl endopeptidase removal of the propeptide, Granzyme A cleaves thioester substrates. Mature human granzyme A shares 71% amino acid sequence identity with mouse granzyme A.

References

Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.
Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.

Entrez Gene IDs

3001 (Human); 14938 (Mouse); 266708 (Rat)

Alternate Names

CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 3.4.21.78