Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2103A
View all Granzyme B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Granzyme B
Gly19-Tyr247
Accession # P10144

Specificity

Detects human Granzyme B in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Granzyme B Antibody

Detection of Granzyme B antibody by Western Blot.

Detection of Granzyme B by Western Blot.

Western blot shows lysates of NK-92 human natural killer lymphoma cell line and NK-RL human natural killer lymphocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB29061) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Granzyme B at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Granzyme B by Western Blot.

Western blot shows lysates of NK‑92 human natural killer lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human Granzyme B Monoclonal Antibody (Catalog # MAB29061) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Granzyme B at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Human Granzyme B Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line and NK‑RL human natural killer lymphocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1-18), a pro peptide (residues 19-20), and a mature chain (residues 21-247) (4-6). The recombinant human (rh) Granzyme B consisting of residues 19-247 was expressed and purified. After being activated by active cathepsin C, rhGranzyme B cleaves a thioester substrate described previously (3).

References

  1. Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. pp. 1549.
  4. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250.
  5. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363.
  6. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Entrez Gene IDs

3002 (Human); 14939 (Mouse)

Gene Symbol

GZMB

UniProt

P10144

Additional Granzyme B Products

Product Documents for Human Granzyme B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Granzyme B Antibody

For research use only

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