Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

Catalog # Availability Size / Price Qty
AF2710
AF2710-SP
Product Details
Citations (1)
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Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2
Gln179-Arg605
Accession # Q96MM7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Heparan Sulfate6-O-Sulfotransferase 2/HS6ST2
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human HS6ST2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2

HS6ST2 is a key enzyme in HS biosynthesis. There are at least three alternatively spliced forms, which include a long form (645 aa, NP_001070656), an intermediate form (605 aa, Q96MM7) with 40 aa deleted between Arg 316 and Asn 357 of the long form, and a short form starting at Met 147 of the intermediate form. Both the long and intermediate forms predict a type II membrane protein, consistent with its Golgi location. The long form of human HS6ST2 shares 94% and 86% aa sequence identities with that of chimpanzee, dog, mouse, rat, and chicken, respectively.

Entrez Gene IDs
90161 (Human)
Alternate Names
EC-1; heparan sulfate 6-O-sulfotransferase 2; HS6ST2

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Citation for Human Heparan Sulfate 6‑O‑Sulfotransferase 2/HS6ST2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiplex genome editing of mammalian cells for producing recombinant heparin
    Authors: BE Thacker, KJ Thorne, C Cartwright, J Park, K Glass, A Chea, BP Kellman, NE Lewis, Z Wang, A Di Nardo, ST Sharfstein, W Jeske, J Walenga, J Hogwood, E Gray, B Mulloy, JD Esko, CA Glass
    Metabolic engineering, 2022-01-14;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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