Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 506230
View all Hepsin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Hepsin
Ser46-Leu417
Accession # P05981

Specificity

Detects human Hepsin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Hepsin Antibody

Hepsin antibody in Human Liver by Immunohistochemistry (IHC-P).

Hepsin in Human Liver.

Hepsin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Hepsin Monoclonal Antibody (Catalog # MAB47761) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Hepsin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB47761 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hepsin

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver but is also present in many other tissues; most notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII and may initiate blood coagulation at the cell surface (4). Hepsin is over-expressed in various human tumors including prostate (5) and is considered to be a biomarker for prostate cancer (6). Recombinant human (rh) Hepsin was expressed as a secreted, soluble protein lacking its cytosolic and transmembrane domains. The D161E and R162K mutations were introduced into the prosequence to improve expression of the rhHepsin.

References

  1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
  2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
  3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
  4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
  5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
  6. Wu, Q. and G. Parry (2007) Front. Biosci. 12:5052.

Alternate Names

HPN, TMPRSS1

Entrez Gene IDs

3249 (Human); 15451 (Mouse); 29135 (Rat)

Gene Symbol

HPN

UniProt

P05981

Additional Hepsin Products

Product Documents for Human Hepsin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hepsin Antibody

For research use only

Citations for Human Hepsin Antibody

Customer Reviews for Human Hepsin Antibody (1)

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  • Human Hepsin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 12/26/2021
    Human Hepsin Antibody MAB47761

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