Human HGK Antibody

Catalog # Availability Size / Price Qty
AF6027
AF6027-SP
Detection of Human HGK by Western Blot.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human HGK Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGK in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HGK
Ser686-Val820
Accession # O95819-4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HGK antibody by Western Blot. View Larger

Detection of Human HGK by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human HGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6027) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HGK at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGK

HGK (Hepatocyte kinase-like/Germinal center like-kinase Kinase; also MAP4K4) is a 140 kDa member of the MSN subfamily, MAP4K family of molecules. It is ubiquitously expressed, and intimately associated with TNF alpha signaling. HGK is activated by TNF‑induced c-Jun activation, and blocks both PPAR gamma expression and glucose-stimulated insulin secretion. Human HGK is 1239 amino acids (aa) in length (Accession # O95819) and contains one protein kinase domain (aa 25‑289) and a CHN region (aa 926‑1213). There are multiple splice variants. The antibody product offered by R&D Systems uses a 130 kDa, 1166 aa isoform as immunogen. It shows a deletion of aa 495‑525 and 570‑623, plus an eight aa insertion after His1112, and a GEV insertion after Ala740.

Long Name
Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase
Entrez Gene IDs
9448 (Human); 26921 (Mouse)
Alternate Names
EC 2.7.11; EC 2.7.11.1; FLH21957; FLJ10410; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HGK; HGKFLJ20373; HPK/GCK-like kinase HGK; KIAA0687; MAP4K4; MAPK/ERK kinase kinase kinase 4; MEK kinase kinase 4; MEKKK 4; MEKKK4; mitogen-activated protein kinase kinase kinase kinase 4; Nck-interacting kinase; NIKFLJ90111

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human HGK Antibody

There are currently no reviews for this product. Be the first to review Human HGK Antibody and earn rewards!

Have you used Human HGK Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review