HGK (Hepatocyte kinase-like/Germinal center like-kinase Kinase; also MAP4K4) is a 140 kDa member of the MSN subfamily, MAP4K family of molecules. It is ubiquitously expressed, and intimately associated with TNF alpha signaling. HGK is activated by TNF‑induced c-Jun activation, and blocks both PPAR gamma expression and glucose-stimulated insulin secretion. Human HGK is 1239 amino acids (aa) in length (Accession # O95819) and contains one protein kinase domain (aa 25‑289) and a CHN region (aa 926‑1213). There are multiple splice variants. The antibody product offered by R&D Systems uses a 130 kDa, 1166 aa isoform as immunogen. It shows a deletion of aa 495‑525 and 570‑623, plus an eight aa insertion after His1112, and a GEV insertion after Ala740.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ser686-Val820
Accession # O95819-4
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human HGK Antibody
Detection of Human HGK by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and PC-3 human prostate cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human HGK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6027) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HGK at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human HGK Antibody
Western Blot
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and PC‑3 human prostate cancer cell line
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HGK
Long Name
Alternate Names
Gene Symbol
UniProt
Additional HGK Products
Product Documents for Human HGK Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HGK Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars