Human HIPK2 Antibody

Catalog # Availability Size / Price Qty
MAB9307-100
MAB9307-SP

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HIPK2 in MCF‑7 Human Cell Line.
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Human HIPK2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HIPK2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 493918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HIPK2
Met765-Ser924
Accession # Q9H2X6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HIPK2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

HIPK2 in MCF‑7 Human Cell Line. HIPK2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HIPK2 Monoclonal Antibody (Catalog # MAB9307) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIPK2

Homeodomain-interacting protein kinase-2 (HIPK2) is a serine/threonine kinase which interacts with transcription factors, including p53, CREB1, SMAD1, SMAD2, RUNX1 and others, and can act as a corepressor or coactivator depending on the transcription factor and subcellular localization.  Post-translational modifications control HIPK2 activity, including phosphorylation, small ubiquitin-like modifier modification, acetylation, and ubiquitination. HIPK2 regulation of cell proliferation makes it a key effector of proliferative diseases including cancer and kidney fibrosis. For example, HIPK2 can repress the proliferative Wnt-1/b-catenin pathway and HIF-1, promote apoptosis by activation of p53 and repression of delta Np63 alpha, MDM2 and CtBP, and its activity is enhanced by anti-cancer drugs such as cisplatin and etoposide.  HIPK2 oncorepession can be blocked by cytoplasmic localization, hypoxia, gene mutation and loss of heterozygosity.

 


References
(Saul VV and Schmitz ML, J Mol Med (Berl). 2013 Sep;91(9):1051-8.)  (Saul VV and Schmitz ML, J Mol Med (Berl). 2013 Sep;91(9):1051-8.)  (D'Orazi G, et al, J Exp Clin Cancer Res. 2012 Aug 13;31:63. )
Long Name
Homeodomain Interacting Protein Kinase 2
Entrez Gene IDs
28996 (Human); 15258 (Mouse); 362342 (Rat)
Alternate Names
DKFZp686K02111; EC 2.7.11.1; FLJ23711; hHIPk2; HIPK2; homeodomain interacting protein kinase 2; homeodomain-interacting protein kinase 2; PRO0593; Stank

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Human HIPK2 Antibody
By Anonymous on 11/11/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: MCF‑7 Human Cell Line Species: Human