Human HIPK2 Antibody

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HIPK2 in MCF‑7 Human Cell Line.
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Human HIPK2 Antibody Summary

Species Reactivity
Detects human HIPK2 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 493918
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HIPK2
Accession # Q9H2X6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry HIPK2 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

HIPK2 in MCF‑7 Human Cell Line. HIPK2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HIPK2 Monoclonal Antibody (Catalog # MAB9307) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HIPK2

Homeodomain-interacting protein kinase-2 (HIPK2) is a serine/threonine kinase which interacts with transcription factors, including p53, CREB1, SMAD1, SMAD2, RUNX1 and others, and can act as a corepressor or coactivator depending on the transcription factor and subcellular localization.  Post-translational modifications control HIPK2 activity, including phosphorylation, small ubiquitin-like modifier modification, acetylation, and ubiquitination. HIPK2 regulation of cell proliferation makes it a key effector of proliferative diseases including cancer and kidney fibrosis. For example, HIPK2 can repress the proliferative Wnt-1/b-catenin pathway and HIF-1, promote apoptosis by activation of p53 and repression of delta Np63 alpha, MDM2 and CtBP, and its activity is enhanced by anti-cancer drugs such as cisplatin and etoposide.  HIPK2 oncorepession can be blocked by cytoplasmic localization, hypoxia, gene mutation and loss of heterozygosity.


(Saul VV and Schmitz ML, J Mol Med (Berl). 2013 Sep;91(9):1051-8.)  (Saul VV and Schmitz ML, J Mol Med (Berl). 2013 Sep;91(9):1051-8.)  (D'Orazi G, et al, J Exp Clin Cancer Res. 2012 Aug 13;31:63. )
Long Name
Homeodomain Interacting Protein Kinase 2
Entrez Gene IDs
28996 (Human); 15258 (Mouse); 362342 (Rat)
Alternate Names
DKFZp686K02111; EC; FLJ23711; hHIPk2; HIPK2; homeodomain interacting protein kinase 2; homeodomain-interacting protein kinase 2; PRO0593; Stank

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Human HIPK2 Antibody
By Anonymous on 11/11/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: MCF‑7 Human Cell Line Species: Human