Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HNF‑4 alpha /NR2A1
Val130-Ser330
Accession # P41235

Specificity

Detects human HNF‑4 alpha /NR2A1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HNF‑4 alpha /NR2A1 Antibody

Detection of Human HNF‑4a/NR2A1 antibody by Western Blot.

Detection of Human HNF‑4 alpha /NR2A1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band for HNF-4a/NR2A1 was detected at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human HNF-4a/NR2A1 antibody by Simple WesternTM.

Detection of Human HNF‑4 alpha /NR2A1 by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HNF-4a/NR2A1 at approximately 60 kDa (as indicated) using 50 µg/mL of Goat Anti-Human HNF-4a/NR2A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4605) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human HNF‑4 alpha /NR2A1 Antibody

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HNF-4 alpha/NR2A1

HNF-4 alpha is a transcription factor that binds DNA as a homodimer. HNF-4 alpha is important in liver, kidney, and intestinal development. It has also been intensely studied as one of a variety of genes responsible for diabetes mellitus. HNF-4 alpha has been shown in knock out mice to be essential for the morphogenic and functional differentiation of hepatocytes. HNF-4 alpha is a dominant regulator of epithelial phenotypes able to drive the mesenchymal-to-epithelial transition when expressed in fibroblasts.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

UniProt

P41235

Additional HNF-4 alpha/NR2A1 Products

Product Documents for Human HNF‑4 alpha /NR2A1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HNF‑4 alpha /NR2A1 Antibody

For research use only

Citations for Human HNF‑4 alpha /NR2A1 Antibody

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail