Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all HNF-6/ONECUT1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HNF-6/ONECUT1
Gln139-Gly283
Accession # Q9UBC0

Specificity

Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human HNF‑6/ONECUT1 Antibody

Detection of Human HNF-6/ONECUT1 antibody by Western Blot.

Detection of Human HNF‑6/ONECUT1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HNF-6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
HNF-6/ONECUT1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

HNF‑6/ONECUT1 in HepG2 Human Cell Line.

HNF-6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of HNF‑6/ONECUT1 in HepG2 (positive) and HDLM-2 (negative) cells.

HNF‑6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Sheep Anti-Human HNF‑6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human HNF‑6/ONECUT1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and HDLM‑2 human Hodgkin's lymphoma cells

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HNF-6/ONECUT1

HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha 1AT, alpha -fetoprotein and HNF-3 beta. Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human.

Long Name

Hepatocyte Nuclear Factor 6

Alternate Names

HNF-6A, HNF6, OC-1, ONECUT1

Entrez Gene IDs

3175 (Human); 15379 (Mouse); 25231 (Rat)

Gene Symbol

ONECUT1

UniProt

Q9UBC0 (Human)

Additional HNF-6/ONECUT1 Products

Product Documents for Human HNF‑6/ONECUT1 Antibody

Certificate of Analysis

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Product Specific Notices for Human HNF‑6/ONECUT1 Antibody

For research use only

Citations for Human HNF‑6/ONECUT1 Antibody

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Protocols

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