Human HNF-6/ONECUT1 Antibody

Catalog # Availability Size / Price Qty
AF6277
AF6277-SP
Detection of Human HNF‑6/ONECUT1 by Western Blot.
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Product Details
Citations (5)
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Human HNF-6/ONECUT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HNF-6/ONECUT1 in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human ONECUT2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HNF-6/ONECUT1
Gln139-Gly283
Accession # Q9UBC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed HepG2 human hepatocellular carcinoma cell line and HDLM‑2 human Hodgkin's lymphoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HNF-6/ONECUT1 antibody by Western Blot. View Larger

Detection of Human HNF‑6/ONECUT1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for HNF-6/ONECUT1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HNF-6/ONECUT1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

HNF‑6/ONECUT1 in HepG2 Human Cell Line. HNF-6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Human HNF-6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry View Larger

Detection of HNF‑6/ONECUT1 in HepG2 (positive) and HDLM-2 (negative) cells. HNF‑6/ONECUT1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cells (positive) and absent in HDLM‑2 human Hodgkin’s lymphoma cells (negative) using Sheep Anti-Human HNF‑6/ONECUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HNF-6/ONECUT1

HNF-6 (also ONECUT1; one "cut" domain family member 1; OC-1) is a monomeric 53-56 kDa member of the One Cut-domain containing class of homeodomain proteins. It is expressed in embryonic hepatic and pancreatic islet anlage, plus adult hepatocytes and intestinal epithelial cells. HNF-6 is a transcriptional activator that is known to interact with Foxa2. Genes regulated by HNF-6 include Glut2, alpha 1AT, alpha -fetoprotein and HNF-3 beta. Human HNF-6 is 465 amino acids (aa) in length. It contains two DNA-binding regions, one classified as a CUT domain (aa 283-369) and another as a homeobox domain (aa 385-444). HNF-6 is both acetylated and phosphorylated, contributing to protein stability and transactivation activity. Over aa 139-283, human HNF 6 shares 99% aa identity with mouse HNF-6. Rat HNF-6 shows a 26 aa insertion after Ala368. Given an overall aa identity of 99% between rat and human, the possibility exists that this insertion may also exist in human.

Long Name
Hepatocyte Nuclear Factor 6
Entrez Gene IDs
3175 (Human); 15379 (Mouse); 25231 (Rat)
Alternate Names
HNF6; HNF-6; HNF-6A; HNF6Ahepatocyte nuclear factor 6, alpha; HNF6hepatocyte nuclear factor 6; HNF-6One cut domain family member 1; OC-1; one cut homeobox 1one cut domain, family member 1; ONECUT1

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Citations for Human HNF-6/ONECUT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Onecut Factors and Pou2f2 Regulate the Distribution of V2 Interneurons in the Mouse Developing Spinal Cord
    Authors: Audrey Harris, Gauhar Masgutova, Amandine Collin, Mathilde Toch, Maria Hidalgo-Figueroa, Benvenuto Jacob et al.
    Frontiers in Cellular Neuroscience
  2. Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits
    Authors: M Toch, A Harris, O Schakman, E Kondratska, JL Boulland, N Dauguet, S Debrulle, C Baudouin, M Hidalgo-Fi, A Gow, JC Glover, F Tissir, F Clotman
    Sci Rep, 2020-01-22;10(1):996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development
    Authors: KU Kabayiza, G Masgutova, A Harris, V Rucchin, B Jacob, F Clotman
    Front Mol Neurosci, 2017-05-26;10(0):157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Vsx1 Transiently Defines an Early Intermediate V2 Interneuron Precursor Compartment in the Mouse Developing Spinal Cord
    Authors: Cédric Francius, María Hidalgo-Figueroa, Stéphanie Debrulle, Barbara Pelosi, Vincent Rucchin, Kara Ronellenfitch et al.
    Frontiers in Molecular Neuroscience
  5. Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord
    Authors: Gauhar Masgutova, Audrey Harris, Benvenuto Jacob, Lynn M. Corcoran, Frédéric Clotman
    Frontiers in Molecular Neuroscience

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