Human HOIP/RNF31 Antibody

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Detection of Human HOIP/RNF31 by Western Blot.
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Product Details
Citations (1)
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Human HOIP/RNF31 Antibody Summary

Species Reactivity
Detects human HOIP/RNF31 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human HOIP/RNF31
Accession # Q96EP0
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HOIP/RNF31 antibody by Western Blot. View Larger

Detection of Human HOIP/RNF31 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human HOIP/RNF31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for HOIP/RNF31 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry HOIP/RNF31 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

HOIP/RNF31 in MCF‑7 Human Cell Line. HOIP/RNF31 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human HOIP/RNF31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8039) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry HOIP/RNF31 antibody in Human Uterus by Immunohistochemistry (IHC-P). View Larger

HOIP/RNF31 in Human Uterus. HOIP/RNF31 was detected in immersion fixed paraffin-embedded sections of human uterus using Sheep Anti-Human HOIP/RNF31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8039) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOIP/RNF31

RNF31 (RING [Really INteresting Gene] Finger Protein 31; also HOIL-1-interacting protein/HOIP, and Zn in-between-RING-finger ubiquitin-associated domain protein/ZIBRA) is a cytoplasmic E3 ubiquitin-protein ligase that is found in breast epithelium and multiple cancer types. Although its predicted MW is 102 kDa, it runs anomalously at 95-135 kDa in SDS-Page. Ubiquitin (Ub) chains are typically thought of as 9 kDa additions to Lys residues of target molecules. The activity associated with Ub addition depends upon the location of the attachment, and the monomeric vs. polymeric nature of the chains. Ub can also be added to N-terminal Met residues by an intracellular complex called LUBAC (Linear Ub chain Assembly Complex). This complex is key to NF kappa B pathway activation. Following exposure of cells to cytokines, LUBAC ubiquitinates NEMO, which subsequently induces IKK beta phosphorylation, I kappa B alpha degradation, and NF kappa B translocation into the nucleus with gene activation. The LUBAC complex contains RNF31, HOIL-1L and sharpin, and it is now known that RNF31 is the catalyst for linear Ub chain formation. Human RNF31 is 1072 amino acids (aa) in length. It contains three consecutive RanBP2-type Zn finger domains (aa 299-438), a utilized phosphoryation site at Ser466, one UBA domain (aa 564-615), and two RING-type Zn finger domains (aa 699-747 and 860-909) with an intervening IBR-type Zn finger region (aa 779-841). There are at least two isoform variants, one that contains a 13 aa substitution for aa 1-164, and another that shows a deletion of aa 73-630 coupled to a deletion of aa 833-841. Human RNF31 shares 87% aa sequence identity with mouse RNF31.

Long Name
HOIL-1-Interacting Protein
Entrez Gene IDs
55072 (Human); 268749 (Mouse); 364386 (Rat)
Alternate Names
EC 6.3.2.-; FLJ10111; FLJ23501; HOIP; MGC19975; ring finger protein 31; RNF31; ZIBRA; ZIBRAHOIL-1-interacting protein; Zinc in-between-RING-finger ubiquitin-associated domain protein

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Citation for Human HOIP/RNF31 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domains
    Authors: PR Elliott, D Leske, J Wagstaff, L Schlicher, G Berridge, S Maslen, F Timmermann, B Ma, R Fischer, SMV Freund, D Komander, M Gyrd-Hanse
    Cell Reports, 2021-10-05;37(1):109777.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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