Human HOXB7 Antibody

Catalog # Availability Size / Price Qty
MAB8040
MAB8040-SP
HOXB7 in MCF‑7 Human Cell Line.
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Human HOXB7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HOXB7 in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 864614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human HOXB7
Met1-Asn123
Accession # P09629
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry HOXB7 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC). View Larger

HOXB7 in MCF‑7 Human Cell Line. HOXB7 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HOXB7 Monoclonal Antibody (Catalog # MAB8040) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HOXB7

HOXB7 (Homeobox protein B7), also called Hox-2C, is a 24 kDa (predicted) member of the Antp homeobox family of transcription factors. It is widely expressed and is overexpressed in many cancers such as melanoma and tumors in lung and breast. It promotes tumorigenesis by promoting activities such as proliferation, invasion, epithelial-mesenchymal transition and angiogenesis.  Human HOXB7 is a 217 amino acid (aa) protein with an Antp-type hexapeptide (aa 126-131) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 137-196). Human HOXB7 aa 1-123 shares 92% aa sequence identity with mouse and rat HOXB7.

Long Name
Homeobox B7
Entrez Gene IDs
3217 (Human); 15415 (Mouse); 497985 (Rat)
Alternate Names
HHO.C1; homeo box 2C; homeo box B7; homeo box c1 protein; homeobox B7; Homeobox protein HHO.C1; Homeobox protein Hox-2C; homeobox protein Hox-B7; HOX2; Hox-2.3; HOX2C; HOX2CHox-2.3; HOXB7

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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