Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 864614
View all HOXB7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HOXB7
Met1-Asn123
Accession # P09629

Specificity

Detects human HOXB7 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human HOXB7 Antibody

HOXB7 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

HOXB7 in MCF‑7 Human Cell Line.

HOXB7 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human HOXB7 Monoclonal Antibody (Catalog # MAB8040) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human HOXB7 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HOXB7

HOXB7 (Homeobox protein B7), also called Hox-2C, is a 24 kDa (predicted) member of the Antp homeobox family of transcription factors. It is widely expressed and is overexpressed in many cancers such as melanoma and tumors in lung and breast. It promotes tumorigenesis by promoting activities such as proliferation, invasion, epithelial-mesenchymal transition and angiogenesis.  Human HOXB7 is a 217 amino acid (aa) protein with an Antp-type hexapeptide (aa 126-131) that mediates heterodimerization, and a DNA-binding homeobox domain (aa 137-196). Human HOXB7 aa 1-123 shares 92% aa sequence identity with mouse and rat HOXB7.

Long Name

Homeobox B7

Alternate Names

HHO.C1, Hox-2.3, HOX2C

Entrez Gene IDs

3217 (Human); 15415 (Mouse); 497985 (Rat)

Gene Symbol

HOXB7

UniProt

P09629

Additional HOXB7 Products

Product Documents for Human HOXB7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HOXB7 Antibody

For research use only

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