Human HSPA8/HSC71 Antibody (3377C)

R&D Systems | Catalog # MAB11819

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3377C
View all HSPA8/HSC71 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSPA8
Asp534-Gly615
Accession # P11142

Specificity

Detects recombinant human HSPA8 in Direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human HSPA8/HSC71 Antibody (3377C)

Detection of Human HSPA8/HSC71 by Western Blot.

Western Blot shows lysates of A431 human epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.01 µg/ml of Rabbit Anti-Human HSPA8/HSC71 Monoclonal Antibody (Catalog # MAB11819) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for HSPA8/HSC71 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human HSPA8/HSC71 by Simple WesternTM.

Left: Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.1 mg/ml. A specific band was detected for HSPA8/HSC71 at approximately 70 kDa (as indicated) using both 1 µg/ml and 5 µg/ml of Rabbit Anti-Human HSPA8/HSC71 Monoclonal Antibody (Catalog # MAB11819) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rabbit Anti-Human HSPA8/HSC71 Monoclonal Antibody (Catalog # MAB11819) tested at 1 µg/ml (blue line) and 5 µg/ml (green line) in the K562 human chronic myelogenous leukemia cell line.

Immersion fixed paraffin-embedded sections of human lung

HSPA8/HSC71 was detected in immersion fixed paraffin-embedded sections of human lung using Rabbit Anti-Human HSPA8/HSC71 Monoclonal Antibody (Catalog # MAB11819) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nucleus. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human HSPA8/HSC71 Antibody (3377C)

Application
Recommended Usage

Immunohistochemistry

0.25-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung

Simple Western

1-5 µg/mL
Sample: K562 chronic myelogenous leukemia cell line

Western Blot

0.01 µg/mL
Sample: A431 human epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSPA8/HSC71

Heat shock cognate 71-kDa protein (HSPA8), also referred to as HSC70, is a member of the heat shock protein 70 (HSP70) family, with a molecular weight of approximately 71 kDa. HSPA8 is an ATP-dependent molecular chaperone ubiquitously expressed in eukaryotic cells and plays a critical role in protein homeostasis. It facilitates the folding of nascent polypeptides, the assembly and disassembly of protein complexes, and the translocation of proteins across membranes. Additionally, HSPA8 is involved in the targeting and degradation of misfolded or damaged proteins via the ubiquitin-proteasome pathway and lysosomal-associated degradation. Beyond its canonical chaperone functions, HSPA8 participates in cellular processes such as autophagy, stress response, and clathrin-mediated endocytosis. Dysregulation of HSPA8 expression or activity has been implicated in various pathological conditions, including neurodegenerative disorders such as Alzheimer's and Parkinson's diseases, where aberrant protein aggregation is a hallmark feature. The multifunctionality and essential cellular roles of HSPA8 underscore its utility as both a biomarker and a potential therapeutic target in a range of diseases.

References

  1. Hartl FU, Hayer-Hartl M. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science. 2002 Mar 8;295(5561):1852-8. doi: 10.1126/science.1068408. PMID: 11884745. 
  2. Kampinga HH, Craig EA. The HSP70 chaperone machinery: J proteins as drivers of functional specificity. Nat Rev Mol Cell Biol. 2010 Aug;11(8):579-92. doi: 10.1038/nrm2941. Erratum in: Nat Rev Mol Cell Biol. 2010 Oct;11(10):750. PMID: 20651708; PMCID: PMC3003299. 
  3. Pustovaya K, Venediktov A, Soldatov V, Kuzmin E, Pokidova K, Gartzeva V, Payushina O, Tsytsarev V, Meglinski I and Piavchenko G (2026) Recent insights into HSP70: proteostasis and beyond. Front. Mol. Biosci. 13:1791536. doi: 10.3389/fmolb.2026.1791536.

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Entrez Gene IDs

3312 (Human)

Gene Symbol

HSPA8

UniProt

P11142 (Human)

Additional HSPA8/HSC71 Products

Product Documents for Human HSPA8/HSC71 Antibody (3377C)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HSPA8/HSC71 Antibody (3377C)

For research use only

Related Research Areas

Heat Shock Proteins (HSPs)

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Protocols

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