Human HTRA1/PRSS11 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human HTRA1/PRSS11 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 275615
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HTRA1/PRSS11
    Gln23-Pro480
    Accession # Q92743
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HTRA1/PRSS11 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB2916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA1/PRSS11 at approximately 51 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HTRA1/PRSS11
HTRA1 is a serine protease encoded by the PRSS11 gene and is expressed in many tissues. HTRA1 regulates the availability of IGFs by cleaving IGFBPs.
  • Long Name:
    High Temperature Requirement Protein-1
  • Entrez Gene IDs:
    5654 (Human)
  • Alternate Names:
    ARMD7; EC 3.4.21; EC 3.4.21.108; High-temperature requirement A serine peptidase 1; HtrA serine peptidase 1; HTRA1; IGFBP5-protease; L56; ORF480; PRSS11; Serine protease 11; serine, 11 (IGF binding)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. HTRA1 promoter variant differentiates polypoidal choroidal vasculopathy from exudative age-related macular degeneration
    Authors: Tsz Kin Ng
    Sci Rep, 2016;6(0):28639.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. Protoporphyrins enhance oligomerization and enzymatic activity of HtrA1 serine protease.
    Authors: Jo, Hakryul, Patterson, Victoria, Stoessel, Sean, Kuan, Chia-Yi, Hoh, Josephin
    PLoS ONE, 2014;9(12):e115362.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  3. Angiographic features of transgenic mice with increased expression of human serine protease HTRA1 in retinal pigment epithelium.
    Authors: Kumar, Sandeep, Berriochoa, Zachary, Ambati, Balamura, Fu, Yingbin
    Invest Ophthalmol Vis Sci, 2014;55(6):3842-50.
  4. Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1.
    Authors: Jacobo, Sarah Me, Deangelis, Margaret, Kim, Ivana K, Kazlauskas, Andrius
    Mol Cell Biol, 2013;33(10):1976-90.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: IP
  5. Down-Regulation of HtrA1 Activates the Epithelial-Mesenchymal Transition and ATM DNA Damage Response Pathways.
    Authors: Wang N, Eckert KA, Zomorrodi AR
    PLoS ONE, 2012;7(6):e39446.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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