Human ICAM-2/CD102 Antibody

Catalog # Availability Size / Price Qty
AF244
AF244-SP
Product Details
Citations (3)
FAQs
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Human ICAM-2/CD102 Antibody Summary

Species Reactivity
Human
Specificity
Detects human ICAM‑2/CD102 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ICAM‑2/CD102
Lys25-Gln223
Accession # CAA33630
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human ICAM‑2/CD102 Fc Chimera (Catalog # 803-I2)
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood monocytes
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Human ICAM-2 Fc Chimera (Catalog # 803-I2, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.

References
  1. Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
  2. Hayflick, J. et al. (1998) Immunologic Res. 17:313.
Long Name
Intercellular Adhesion Molecule 2
Entrez Gene IDs
3384 (Human); 15896 (Mouse)
Alternate Names
CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2

Product Datasheets

Citations for Human ICAM-2/CD102 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process
    Authors: SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki
    Cell Rep, 2019;29(6):1610-1620.e4.
    Species: Human
    Sample Types: Whole Cells
    Applications: IF
  2. ICAM-2 confers a non-metastatic phenotype in neuroblastoma cells by interaction with alpha-actinin.
    Authors: Feduska J, Aller S, Garcia P, Cramer S, Council L, van Waardenburg R, Yoon K
    Oncogene, 2015;34(12):1553-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
    Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
    Arthritis Rheum., 2007;56(6):1817-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Secondary Antibodies

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