Human ICAM-2/CD102 Antibody

Catalog #: AF244
3 Citations Datasheet / COA / SDS

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AF244
AF244-SP

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Product Details

Citations (3)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICAM-2/CD102 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICAM‑2/CD102 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ICAM-1 and rhICAM-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ICAM‑2/CD102
Lys25-Gln223
Accession # CAA33630

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICAM‑2/CD102 Fc Chimera (Catalog # 803-I2)

Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood monocytes

Adhesion Blockade

The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 10⁴ cells/well) to immobilized Recombinant Human ICAM-2 Fc Chimera (Catalog # 803-I2, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-2/CD102

Intercellular Adhesion Molecule-2 (ICAM-2, CD102), a member of the immunoglobulin superfamily, binds the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). ICAM-2 is constitutively expressed at high levels on vascular endothelial cells and lymphohematopoietic cells. ICAM-2 mediated adhesion has been shown to provide a co-stimulatory signal for T cell aggregation, NK cytotoxicity and NK cell migration.

References

Somersalo, K. et al. (1995) J. Biol. Chem. 270:8629.
Hayflick, J. et al. (1998) Immunologic Res. 17:313.

Long Name

Intercellular Adhesion Molecule 2

Entrez Gene IDs

3384 (Human); 15896 (Mouse)

Alternate Names

CD102 antigen; CD102; ICAM2; ICAM-2; intercellular adhesion molecule 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human ICAM-2/CD102 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Hepatocytes Delete Regulatory T Cells by Enclysis, a CD4+ T Cell Engulfment Process
Authors: SP Davies, GM Reynolds, AL Wilkinson, X Li, R Rose, M Leekha, YS Liu, R Gandhi, E Buckroyd, J Grove, NM Barnes, RC May, SG Hubscher, DH Adams, Y Huang, O Qureshi, Z Stamataki
Cell Rep, 2019-11-05;29(6):1610-1620.e4.
Species: Human
Sample Types: Whole Cells
Applications: IF
ICAM-2 confers a non-metastatic phenotype in neuroblastoma cells by interaction with alpha-actinin.
Authors: Feduska J, Aller S, Garcia P, Cramer S, Council L, van Waardenburg R, Yoon K
Oncogene, 2014-04-07;34(12):1553-62.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis Rheum., 2007-06-01;56(6):1817-26.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization