Human Iduronate 2-Sulfatase/IDS DuoSet ELISA

Catalog #: DY2449-05 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
Ancillary Products Available

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Human Iduronate 2-Sulfatase / IDS ELISA Standard Curve
1 Image
Product Details
Supplemental Products

Human Iduronate 2-Sulfatase/IDS DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Iduronate 2-Sulfatase / IDS. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Iduronate 2-Sulfatase / IDS ELISA Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Iduronate 2-Sulfatase/IDS

Sulfatases belong to a highly conserved family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. Among 18 human sulfatase genes identified, about 1/3 are found in lysosomes and serve to remove sulfate from glycosaminoglycans (GAGs), glycopeptides and glycolipids (GLs). The remaining sulfatases are found in microsomes, endoplasmic reticulum (ER), Golgi, at the cell surface, or secreted and are active against hydroxysteroids, heparin or unidentified substrates. Genetic deficiencies of individual sulfatases reveal the critical importance of these enzymes in the metabolism of specific forms of sulfate. Deficiencies of lysosomal sulfatases that act on GAGs and GLs account for mucopolysaccharidoses (MPS) and metachromatic leukodystrophy, respectively. Deficiencies in non-lysosomal sulfatases, arylsulfases C and E, cause X-linked ichthyosis (XLI) and chondrodysplasia punctata (CDPX), respectively.

Entrez Gene IDs:
3423 (Human); 15931 (Mouse)
Alternate Names:
Alpha-L-iduronate sulfate sulfatase; EC; IDS; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate 2-sulfatase; idursulfase; MPS2; S; SIDS


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human Iduronate 2-Sulfatase/IDS DuoSet ELISA

There are currently no reviews for this product. Be the first to review Human Iduronate 2-Sulfatase/IDS DuoSet ELISA and earn rewards!

Have you used Human Iduronate 2-Sulfatase/IDS DuoSet ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review