Human IFN-alpha 2/IFNA2 Antibody

Catalog # Availability Size / Price Qty
IFN‑ alpha 2/IFNA2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ alpha 2/IFNA2 Antibody.
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Product Details
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Human IFN-alpha 2/IFNA2 Antibody Summary

Species Reactivity
Detects human IFN‑ alpha 2/IFNA2 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 967305
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha 2/IFNA2
Accession # P01563
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Measured by its ability to neutralize IFN‑ alpha 2/IFNA2 inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND50) is typically 0.6-3.6 ng/mL in the presence of 0.25 ng/mL Recombinant Human IFN‑ alpha 2/IFNA2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ alpha 2/IFNA2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ alpha 2/IFNA2 Antibody. View Larger

IFN‑ alpha 2/IFNA2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ alpha 2/IFNA2 Antibody. Recombinant Human IFN-alpha 2/IFNA2 reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-alpha 2/IFNA2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-alpha 2/IFNA2 Monoclonal Antibody (Catalog # MAB93452). The ND50 is typically 0.6-3.6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFNA2), also known as leukocyte interferon, is a type I interferon  produced by macrophages,  CD8 resting T cells, tonsillar NK cells, germinal center B cells, as well as epithelial cells, tumor cells and melanocytes. IFN alpha has at least nine different isoforms.

IFNA2 is a ligand for a cell surface receptor with two subunits, IFN-alpha R2 (ligand binding) and IFN-alpha R1 (ligand binding and signal transduction). IFNA2 has both antiviral and immunomodulatory activities on target cells.  Intranasal administration has been shown to reduce viral load in subjects with the common cold. While IFNA2 is a prognostic factor for hepatocellular carcinoma, Interferon alpha 2b, has been used to treat melanoma, renal cell carcinoma, chronic myelogenous leukemia and hepatitis C.

  1. Chen, Zy et al. (2014) Br. J. Cancer 110:733.
  2. Burchert, A. et al. (2015) Leukemia 29:1331.
  3. Perrakis, A. et al. (2011) Transplant Proc. 43:3824.
  4. Akaza, H. et al. (2011) Jpn. J. Clin. Oncol. 41:1023.
Long Name
Interferon alpha 2
Entrez Gene IDs
3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names
alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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