Human IFN-alpha 2/IFNA2 Antibody

Catalog # Availability Size / Price Qty
MAB93452-100
MAB93452-SP
IFN‑ alpha 2/IFNA2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ alpha 2/IFNA2 Antibody.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human IFN-alpha 2/IFNA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN‑ alpha 2/IFNA2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 967305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line transfected with human IFN‑ alpha 2/IFNA2
Met1-Glu188
Accession # P01563
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IFN‑ alpha 2/IFNA2 inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. The Neutralization Dose (ND50) is typically 0.6-3.6 ng/mL in the presence of 0.25 ng/mL Recombinant Human IFN‑ alpha 2/IFNA2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization IFN‑ alpha 2/IFNA2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ alpha 2/IFNA2 Antibody. View Larger

IFN‑ alpha 2/IFNA2 Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ alpha 2/IFNA2 Antibody. Recombinant Human IFN-alpha 2/IFNA2 reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-alpha 2/IFNA2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-alpha 2/IFNA2 Monoclonal Antibody (Catalog # MAB93452). The ND50 is typically 0.6-3.6 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFNA2), also known as leukocyte interferon, is a type I interferon  produced by macrophages,  CD8 resting T cells, tonsillar NK cells, germinal center B cells, as well as epithelial cells, tumor cells and melanocytes. IFN alpha has at least nine different isoforms.

IFNA2 is a ligand for a cell surface receptor with two subunits, IFN-alpha R2 (ligand binding) and IFN-alpha R1 (ligand binding and signal transduction). IFNA2 has both antiviral and immunomodulatory activities on target cells.  Intranasal administration has been shown to reduce viral load in subjects with the common cold. While IFNA2 is a prognostic factor for hepatocellular carcinoma, Interferon alpha 2b, has been used to treat melanoma, renal cell carcinoma, chronic myelogenous leukemia and hepatitis C.

References
  1. Chen, Zy et al. (2014) Br. J. Cancer 110:733.
  2. Burchert, A. et al. (2015) Leukemia 29:1331.
  3. Perrakis, A. et al. (2011) Transplant Proc. 43:3824.
  4. Akaza, H. et al. (2011) Jpn. J. Clin. Oncol. 41:1023.
Long Name
Interferon alpha 2
Entrez Gene IDs
3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names
alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human IFN-alpha 2/IFNA2 Antibody

There are currently no reviews for this product. Be the first to review Human IFN-alpha 2/IFNA2 Antibody and earn rewards!

Have you used Human IFN-alpha 2/IFNA2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review