Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 995728
View all IFN-epsilon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IFN-epsilon
Leu22-Arg208
Accession # Q86WN2

Specificity

Detects human IFN-epsilon in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IFN-epsilon Antibody

IFN-epsilon antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

IFN-epsilon in HeLa Human Cell Line.

IFN-epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human IFN-epsilon Monoclonal Antibody (Catalog # MAB91472) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human IFN-epsilon Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-epsilon

Interferon-epsilon (IFNe) is a type I interferon with expression limited to the brain, lung, intestine and reproductive tract. It has been proposed that unlike other type I interferons, IFNe plays a role in mucosal immunity and protects against pathogens including chlamidia, HIV and herpes simplex virus 2. Its expression is upregulated by TNF alpha. Unlike other type-1 interferons, IFNe is also hormonally regulated, and mouse and human IFNe have been shown to have a binding site in their promoters for progesterone receptor. Epithelial cells isolated from uterine endometrium have higher IFNee expression in the proliferative phase, when estrogen levels are highest. IFNe is the only IFN isoform constituitively expressed in HeLa cells.

Long Name

Interferon epsilon

Alternate Names

Ifn-tau-11, IFNE, IFNepsilon, IFNT1, INFE1

Entrez Gene IDs

338376 (Human); 230405 (Mouse); 298225 (Rat)

Gene Symbol

IFNE

UniProt

Q86WN2

Additional IFN-epsilon Products

Product Documents for Human IFN-epsilon Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IFN-epsilon Antibody

For research use only

Related Research Areas

Interferons

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