Human IFN-epsilon Antibody Summary
Accession # Q86WN2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN-epsilon in HeLa Human Cell Line. IFN-epsilon was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human IFN-epsilon Monoclonal Antibody (Catalog # MAB91472) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interferon-epsilon (IFNe) is a type I interferon with expression limited to the brain, lung, intestine and reproductive tract. It has been proposed that unlike other type I interferons, IFNe plays a role in mucosal immunity and protects against pathogens including chlamidia, HIV and herpes simplex virus 2. Its expression is upregulated by TNF alpha. Unlike other type-1 interferons, IFNe is also hormonally regulated, and mouse and human IFNe have been shown to have a binding site in their promoters for progesterone receptor. Epithelial cells isolated from uterine endometrium have higher IFNee expression in the proliferative phase, when estrogen levels are highest. IFNe is the only IFN isoform constituitively expressed in HeLa cells.
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