Human IGFBP-rp1/IGFBP-7 Antibody

Catalog # Availability Size / Price Qty
MAB1334-SP
MAB1334-500
MAB1334-100
Human IGFBP‑rp1/IGFBP‑7 ELISA Standard Curve.
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Product Details
Citations (3)
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Human IGFBP-rp1/IGFBP-7 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGFBP‑rp1/IGFBP-7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human IGFBP-1, -2, -3, -4, -5, or -6 is observed.
Source
Monoclonal Mouse IgG1 Clone # 192520
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGFBP‑rp1/IGFBP-7
Asp30-Arg277
Accession # AAA16187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IGFBP-rp1/IGFBP‑7 (Catalog # 1334-B7)
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IGFBP‑rp1/IGFBP‑7 Monoclonal Antibody (Catalog # MAB13342).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IGFBP-rp1/IGFBP-7 DuoSet ELISA Kit (Catalog # DY1334-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human IGFBP‑rp1/IGFBP‑7 ELISA Standard Curve. Recombinant Human IGFBP-rp1/IGFBP-7 protein was serially diluted 2-fold and captured by Mouse Anti-Human IGFBP-rp1/IGFBP-7 Monoclonal Antibody (Catalog # MAB13342) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IGFBP-rp1/IGFBP-7 Monoclonal Antibody (Catalog # MAB1334) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-rp1/IGFBP-7

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) precursor protein with a putative 26 aa signal peptide. Mature
IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal
immunoglobulin‑like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP1 through 5. Human and mouse IGFBP-rP1 are highly homologous. Human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP-rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP‑rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1-3).

References
  1. Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
  2. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
  3. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.
Long Name
Insulin-like Growth Factor Binding Protein Related Protein 1
Entrez Gene IDs
3490 (Human); 29817 (Mouse)
Alternate Names
angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor

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Citations for Human IGFBP-rp1/IGFBP-7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IGFBP7 Induces Differentiation and Loss of Survival of Human Acute Myeloid Leukemia Stem Cells without Affecting Normal Hematopoiesis
    Authors: HJMP Verhagen, N van Gils, T Martiañez, A van Rhenen, A Rutten, F Denkers, DC de Leeuw, MA Smit, ML Tsui, LLE de Vos Klo, RX Menezes, M Çil, MGM Roemer, E Vermue, S Heukelom, S Zweegman, JJWM Janssen, GJ Ossenkoppe, GJ Schuurhuis, L Smit
    Cell Rep, 2018-12-11;25(11):3021-3035.e5.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
    Authors: Gunawardana CG, Kuk C, Smith CR
    J. Proteome Res., 2009-10-01;8(10):4705-13.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  3. Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach.
    Authors: Gronborg M, Kristiansen TZ, Iwahori A, Chang R, Reddy R, Sato N, Molina H, Jensen ON, Hruban RH, Goggins MG, Maitra A, Pandey A
    Mol. Cell Proteomics, 2005-10-08;5(1):157-71.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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