Human Ig lambda C Domain Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human Immunoglobulin Lambda Constant Region by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Immunoglobulin lambda constant region at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Preparation and Storage

Background: Ig Lambda C Domain

Immunoglobulin lambda constant region is a Ke-Oz- component of immunoglobulin (Ig) lambda light chains and is found C-terminal to the joining region of the light chain. IGLC2 is 105 amino acids (aa) in length. It contains one Ig-like domain (aa 7-100) and generates a disulfide bond with an IgH chain via Cys104. One potential variant shows a three aa substitution for aa 7-9. The closest mouse  lambda ortholog shares 61% aa identity with full-length human IGLC2. IGLC2 shares 93 - 98 % aa identity with IGLC1, IGLC3 and IGLC7.

Product Datasheets