Human Ig lambda C Domain Antibody

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Detection of Human Immunoglobulin Lambda Constant Region by Western Blot.
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Human Ig lambda C Domain Antibody Summary

Species Reactivity
Detects recombinant human IGLC2 in direct ELISAs and Western blots. Detects endogenous Immunoglobulin lambda constant region in natural samples by Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human IGLC2
Accession # P0CG05
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Immunoglobulin Lambda Constant Region antibody by Western Blot. View Larger

Detection of Human Immunoglobulin Lambda Constant Region by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF Membrane was probed with 1 µg/mL of Human Ig lambda C Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6525) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Immunoglobulin lambda constant region at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ig Lambda C Domain

Immunoglobulin lambda constant region is a Ke-Oz- component of immunoglobulin (Ig) lambda light chains and is found C-terminal to the joining region of the light chain. IGLC2 is 105 amino acids (aa) in length. It contains one Ig-like domain (aa 7-100) and generates a disulfide bond with an IgH chain via Cys104. One potential variant shows a three aa substitution for aa 7-9. The closest mouse  lambda ortholog shares 61% aa identity with full-length human IGLC2. IGLC2 shares 93 - 98 % aa identity with IGLC1, IGLC3 and IGLC7.

Long Name
Immunoglobulin lambda C Domain
Entrez Gene IDs
3538 (Human)
Alternate Names
Ig Lambda C Domain; IGLC; IGLC2; immunoglobulin lambda constant 2 (Kern-Oz- marker); MGC20392; MGC45681

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