Human IKK beta Antibody

Catalog # Availability Size / Price Qty
AF4535
AF4535-SP
Human IKK beta  Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human IKK beta Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human IKK beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IKK alpha, IKK gamma, or IKK epsilon.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IKK beta
Gly374-Ser756
Accession # O14920
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human IKK beta  antibody by Western Blot. View Larger

Detection of Human IKK beta by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Raji human Burkitt's lymphoma cell line, and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human IKK beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4535) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IKK beta

I kappa B kinase beta (IKK beta ) is also known as IKBKB and IKK2. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappa B, resulting in the release and nuclear translocation of active NF-kappa B. Like IKK alpha, IKK beta contains kinase, leucine zipper, and helix‑loop‑helix domains. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha /IKK beta heterodimers.

Long Name
IkB Kinase beta
Entrez Gene IDs
3551 (Human); 16150 (Mouse); 84351 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.10; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK beta; IKK2; IKK2FLJ38368; IKK-B; IKK-betaFLJ36218; IKKBFLJ40509; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; NFKBIKBFLJ33771; Nuclear factor NF-kappa-B inhibitor kinase beta

Product Datasheets

Citations for Human IKK beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages.
    Authors: Awuh J, Haug M, Mildenberger J, Marstad A, Do C, Louet C, Stenvik J, Steigedal M, Damas J, Halaas O, Flo T
    Proc Natl Acad Sci U S A, 2015;112(31):E4272-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-kappaappa B signaling induced by tumor necrosis factor-alpha in human A549 lung epithelial cells.
    Authors: Xu J, Wu F, Tian D, Wang J, Zheng Z, Xia N
    PLoS ONE, 2014;9(6):e100787.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human IKK beta Antibody
By Anonymous on 01/16/2020
Application: ELISA Sample Tested: PDX sample Species: Human