Human IL-11 R alpha Antibody

Catalog # Availability Size / Price Qty
MAB1977
MAB1977-SP
Product Details
Citations (2)
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Human IL-11 R alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-11 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rmIL-11 R alpha or recombinant human IL‑6 R alpha is observed. In Western blots, 25% cross-reactivity with recombinant mouse (rm) IL-11 R alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 473143
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-11 R alpha
Cys26-Val363
Accession # Q14626
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑11 R alpha Fc Chimera (Catalog # 1977-MR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-11 R alpha

Human interleukin 11 receptor alpha (IL-11 R alpha ) is 150 kDa type I transmembrane protein in the hematopoietin cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 422 amino acid (aa) residue precursor type I membrane protein, containg a signal peptide followed by extracellular (ECD), transmembrane and cytoplasmic domains. The extracellular region which is closely related to that of IL-6 R alpha and CNTF R alpha contains an immunoglobulin-like domain and two fibronectin-type III-like domains. Within the ECD, human IL-11 R alpha shares 85% and 83% aa sequence identity with mouse and rat IL-11 R alpha, respectively (1‑3). Alternate splicing generates an isoform of human IL-11 R alpha which lacks the cytoplasmic domain. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha, together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist (3‑6).

References
  1. Cherel, M. et al. (1995) Blood 86:2534.
  2. Schleinkofer, K. et al. (2001) J. Mol. Biol. 306:263.
  3. Nandurkar, H.H. et al. (1996) Oncogene 12:585.
  4. Taga, T. et al. (1997) Annu. Rev. Immuno. 15:797.
  5. Davidson, A.J. et al. (1997) Stem Cells 15:119.
  6. Curtis, D.J. et al. (1997) Blood 90:4403.
Long Name
Interleukin 11 Receptor alpha
Entrez Gene IDs
3590 (Human); 16157 (Mouse)
Alternate Names
AI314697; GP130; IL-11 R alpha; IL11R alpha; IL11RA; IL-11Ra; Il11ra2; NR1

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Citations for Human IL-11 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Bovine lactoferricin-induced anti-inflammation is, in part, via up-regulation of interleukin-11 by secondary activation of STAT3 in human articular cartilage.
    Authors: Yan, Dongyao, Kc, Ranjan, Chen, Di, Xiao, Guozhi, Im, Hee-Jeon
    J Biol Chem, 2013-09-13;288(44):31655-69.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inactivation of IL11 signaling causes craniosynostosis, delayed tooth eruption, and supernumerary teeth.
    Authors: Nieminen P, Morgan NV, Fenwick AL, Parmanen S, Veistinen L, Mikkola ML, van der Spek PJ, Giraud A, Judd L, Arte S, Brueton LA, Wall SA, Mathijssen IM, Maher ER, Wilkie AO, Kreiborg S, Thesleff I
    Am. J. Hum. Genet., 2011-07-15;89(1):67-81.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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