Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IL-12 R beta 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 1
Cys24-Glu540
Accession # P42701

Specificity

Detects human IL-12 R beta 1 in direct ELISAs and Western blots. In these formats, less than 2% cross‑reactivity with recombinant mouse IL‑12 R beta 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human IL‑12 R beta 1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑12 R beta 1 Fc Chimera (Catalog # 839-B1)

Reviewed Applications

Read 1 review rated 4 using AF839 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12 R beta 1

Interleukin 12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I membrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) residue protein with a putative 23 aa residue signal peptide that is cleaved to generate the mature protein with a 522 aa residue extracellular domain, a 25 aa residue transmembrane domain and a 92 aa residue cytoplasmic region. Expression of IL-12 R beta 1 has been detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.

References

  1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

P42701

Additional IL-12 R beta 1 Products

Product Documents for Human IL‑12 R beta 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL‑12 R beta 1 Antibody

For research use only

Citations for Human IL‑12 R beta 1 Antibody

Customer Reviews for Human IL‑12 R beta 1 Antibody (1)

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  • Human IL-12 R beta 1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 02/13/2023
    working good for the detection of human IL12 R beta 1 in the serum and plasma samples (1:5 dilution).
    Human IL‑12 R beta 1 Antibody AF839

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