Human IL-26/AK155 Antibody

Catalog #: MAB13751
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13751
MAB13751-SP

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Detection of IL‑26/AK155 in Human PBMCs by Flow Cytometry.

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-26/AK155 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-26/AK155 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 510414

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL-26/AK155
Lys22-Gln171
Accession # Q9NPH9.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL‑26/AK155 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of IL‑26/AK155 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with PMA and Calcium ionomycin, then stained with Mouse Anti-Human IL-26/AK155 Monoclonal Antibody (Catalog # MAB13751, dark orange filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-26/AK155

IL-26, also named AK155, belongs to the family of IL-10-related cytokines.

Long Name

Interleukin 26

Entrez Gene IDs

55801 (Human)

Alternate Names

AK155; AK155IL-26interleukin-26; IL26; IL-26; interleukin 26; Protein AK155

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human IL-26/AK155 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes.
Authors: Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C
J Immunol, 2015-03-18;194(8):3697-712.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry