Intracellular Staining by Flow Cytometry
Detection of IL‑26/AK155 in Human PBMCs by Flow Cytometry. |
Human peripheral blood mononuclear cells were unstimulated (light orange filled histogram) or treated with PMA and Calcium ionomycin, then stained with Mouse Anti-Human IL‑26/AK155 Monoclonal Antibody (Catalog # MAB13751, dark orange filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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