Human IL-36 beta /IL-1F8 Antibody

Catalog # Availability Size / Price Qty
MAB1099
MAB1099-SP
Product Details
Citations (1)
FAQs
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Reviews

Human IL-36 beta /IL-1F8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑36 beta /IL‑1F8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1ra, rhIL-18, rhIL-36Ra, rhIL-36 alpha, rhIL-1F7 or recombinant mouse IL-36 beta is observed.
Source
Monoclonal Mouse IgG1 Clone # 162601
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑36 beta /IL‑1F8
Met1-Glu157
Accession # NP_775270
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑36 beta /IL‑1F8 (Catalog # 1099-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 beta/IL-1F8

Human interleukin 1 family member #8 [IL-1F8; also named IL-36 beta FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3, 6). IL-1 family members include IL‑1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Two alternatively spliced transcript variants encode distinct (164 or 157 residues) protein isoforms that differ in their C-terminal 70 amino acid (aa) residues have been reported (3). IL-36 beta isoform 2 is synthesized as a 157 aa protein that contains no signal sequence and no prosegment (1‑2). Unlike IL-36 beta isoform 1 which lacks potential N-linked glycosylation sites, isoform 2 contains one potential N-linked glycosylation site in its unique C-terminus. IL-36 beta is reported to be actively secreted (1). Human IL-36 beta isoform 2 shares 61% aa identity with mouse IL-1 ra, a 183 aa form of IL-36 beta. Within the IL-1 family, IL-36 beta shares 30%, 32%, 37%, 46%, 34%, 45% and 28% aa sequence identity with IL-1 ra, IL-1 beta, IL-36Ra, IL-36 alpha, F7, IL-36 gamma and F10, respectively. Cells reported to express IL-36 beta include resting and activated monocytes and B cells (1, 4). The receptor for IL-36 beta is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-36 beta, along with IL-36 alpha and IL-36 gamma, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

 

References
  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Entrez Gene IDs
27177 (Human); 69677 (Mouse)
Alternate Names
family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta

Product Datasheets

Citation for Human IL-36 beta /IL-1F8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-1F5, -F6, -F8, and -F9: A Novel IL-1 Family Signaling System That Is Active in Psoriasis and Promotes Keratinocyte Antimicrobial Peptide Expression.
    Authors: Johnston A, Xing X, Guzman AM, Riblett M, Loyd CM, Ward NL, Wohn C, Prens EP, Wang F, Maier LE, Kang S, Voorhees JJ, Elder JT, Gudjonsson JE
    J. Immunol., 2011;186(4):2613-22.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen

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Isotype Controls

Mouse IgG1 Isotype Control

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Reconstitution Buffers

Secondary Antibodies

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