Human IL-36Ra/IL-1F5 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human IL-36Ra / IL-1F5 ELISA Standard Curve
Preparation and Storage
Interleukin 36 Receptor Antagonist (IL-36Ra), also known as IL-1F5, FIL1 delta, IL-1HY1, IL-1H3, and IL-1L1, is a member of the IL-1 family of proteins. All family members show a 12 beta-strand, beta-trefoil configuration, and all are believed to have arisen from a common ancestral gene that underwent multiple duplications. The human IL-36Ra gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of IL-1ra. Cells reported to express IL-36Ra include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus parietal and chief cells.
Citations for Human IL-36Ra/IL-1F5 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis
Authors: X Qin, M Liu, S Zhang, C Wang, T Zhang
Sample Types: Serum
Interleukin-36 cytokines alter the intestinal microbiome and can protect against obesity and metabolic dysfunction
Authors: E Giannoudak, YE Hernandez-, K Mulfaul, SL Doyle, E Hams, PG Fallon, A Mat, D O'Shea, M Kopf, AE Hogan, PT Walsh
Nat Commun, 2019;10(1):4003.
Sample Types: Serum
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Average Rating: 3 (Based on 1 Review)
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We tried to run human EDTA-plasma and serum samples with this analysis. The majority of samples however ended up below the detection limit. The blank was unusually high (circa 300mOD) and the highest control was always under 2000mOD.