Human IL-36Ra/IL-1F5 DuoSet ELISA

R&D Systems | Catalog # DY1275-05

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

93.8-6000 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human IL-36Ra/IL-1F5 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all IL-36Ra/IL-1F5 ELISA Kits »

Product Summary for Human IL-36Ra/IL-1F5 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-36Ra / IL-1F5. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human IL-36Ra/IL-1F5 DuoSet ELISA

Human IL-36Ra / IL-1F5 ELISA Standard Curve

Human IL-36Ra / IL-1F5 ELISA Standard Curve

Kit Contents for Human IL-36Ra/IL-1F5 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-36Ra/IL-1F5

Interleukin 36 Receptor Antagonist (IL-36Ra), also known as IL-1F5, FIL1 delta, IL-1HY1, IL-1H3, and IL-1L1, is a member of the IL-1 family of proteins. All family members show a 12 beta-strand, beta-trefoil configuration, and all are believed to have arisen from a common ancestral gene that underwent multiple duplications. The human IL-36Ra gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of IL-1ra. Cells reported to express IL-36Ra include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus parietal and chief cells.

Long Name

Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5

Alternate Names

FIL1 delta, IL-1HY1, IL-1L1, IL-1RP3, IL1F5, IL36Ra, IL36RN

Entrez Gene IDs

26525 (Human); 54450 (Mouse)

Gene Symbol

IL36RN

Additional IL-36Ra/IL-1F5 Products

Product Documents for Human IL-36Ra/IL-1F5 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-36Ra/IL-1F5 DuoSet ELISA

For research use only

Related Research Areas

IL-1 Family

Citations for Human IL-36Ra/IL-1F5 DuoSet ELISA

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  • Human IL-36Ra/IL-1F5 DuoSet ELISA
    Name: Jonatan Dereke
    Sample Tested: EDTA Plasma and Serum
    Verified Customer | Posted 10/01/2018
    We tried to run human EDTA-plasma and serum samples with this analysis. The majority of samples however ended up below the detection limit. The blank was unusually high (circa 300mOD) and the highest control was always under 2000mOD.
    Human IL-36Ra/IL-1F5 DuoSet ELISA DY1275-05

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