Human IL-36Ra/IL-1F5 DuoSet ELISA

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Human IL-36Ra / IL-1F5 ELISA Standard Curve
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Product Details
Citations (3)
Supplemental Products
Reviews (1)

Human IL-36Ra/IL-1F5 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
93.8 - 6,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-36Ra / IL-1F5. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human IL-36Ra / IL-1F5 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-36Ra/IL-1F5

Interleukin 36 Receptor Antagonist (IL-36Ra), also known as IL-1F5, FIL1 delta, IL-1HY1, IL-1H3, and IL-1L1, is a member of the IL-1 family of proteins. All family members show a 12 beta-strand, beta-trefoil configuration, and all are believed to have arisen from a common ancestral gene that underwent multiple duplications. The human IL-36Ra gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of IL-1ra. Cells reported to express IL-36Ra include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus parietal and chief cells.

Long Name:
Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5
Entrez Gene IDs:
26525 (Human); 54450 (Mouse)
Alternate Names:
FIL1 delta; FIL1; FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta); FIL1DIL-1 delta; IL1F5 (Canonical product IL-1F5a); IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; interleukin 1 family, member 5 (delta); interleukin 1, delta; Interleukin 36 Receptor Antagonist; interleukin-1 family member 5; Interleukin-1 HY1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; Interleukin-36 Receptor Antagonist

Citations for Human IL-36Ra/IL-1F5 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections
    Authors: X Gao, PKS Chan, GCY Lui, DSC Hui, IM Chu, X Sun, MS Tsang, BCL Chan, CW Lam, CK Wong
    Cell Death & Disease, 2021-01-07;12(1):53.
    Species: Human
    Sample Types: Serum
  2. Interleukin-36 cytokines alter the intestinal microbiome and can protect against obesity and metabolic dysfunction
    Authors: E Giannoudak, YE Hernandez-, K Mulfaul, SL Doyle, E Hams, PG Fallon, A Mat, D O'Shea, M Kopf, AE Hogan, PT Walsh
    Nat Commun, 2019-09-05;10(1):4003.
    Species: Human
    Sample Types: Serum
  3. The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis
    Authors: X Qin, M Liu, S Zhang, C Wang, T Zhang
    Cytokine, 2019-03-01;117(0):84-90.
    Species: Human
    Sample Types: Serum


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Reviews for Human IL-36Ra/IL-1F5 DuoSet ELISA

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Human IL-36Ra/IL-1F5 DuoSet ELISA
By Jonatan Dereke on 10/01/2018
Sample Tested: EDTA Plasma,Serum

We tried to run human EDTA-plasma and serum samples with this analysis. The majority of samples however ended up below the detection limit. The blank was unusually high (circa 300mOD) and the highest control was always under 2000mOD.