Human IL-6/IL-6R alpha Complex DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human IL-6 / IL-6 R alpha Complex ELISA Standard Curve
Preparation and Storage
Background: IL-6/IL-6R alpha Complex
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
Citations for Human IL-6/IL-6R alpha Complex DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Activation of STAT3 signaling is mediated by TFF1 silencing in gastric neoplasia
Authors: M Soutto, Z Chen, AA Bhat, L Wang, S Zhu, A Gomaa, A Bates, NS Bhat, D Peng, A Belkhiri, MB Piazuelo, MK Washington, XC Steven, R Peek, W El-Rifai
Nat Commun, 2019;10(1):3039.
Sample Types: Cell Culture Supernates
The balance ofï¿½Interleukin (IL)-6, IL-6:soluble IL-6 receptor (IL-6R) and IL-6:sIL-6R:sgp130 complexes allows simultaneous classic and trans-signaling
Authors: P Baran, S Hansen, GH Waetzig, M Akbarzadeh, L Lamertz, HJ Huber, MR Ahmadian, JM Moll, J Scheller
J. Biol. Chem., 2018;0(0):.
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Reviews for Human IL-6/IL-6R alpha Complex DuoSet ELISA
Average Rating: 4 (Based on 2 Reviews)
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We used this kit for the quantification of IL-6R in human serum and plasma. Works very well and well-described protocol.
Works as expected