Human IL-8/CXCL8 Biotinylated Antibody

(29 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant
    porcine IL-8/CXCL8, recombinant rat (rr) CINC-2 alpha, and rrCINC-2 beta is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-8/CXCL8
    Ser28-Ser99
    Accession # P10145
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL-8/CXCL8 (Catalog # 208-IL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
    • Human IL-8/CXCL8 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human IL-8/CXCL8 Antibody (Catalog # MAB208)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human IL-8/CXCL8 Biotinylated Antibody (Catalog # BAF208)
  • ELISA Standard
     
    Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed PMA-, ionomycin- and monensin-activated human peripheral blood mononuclear cells (PBMCs) using Human IL-8/CXCL8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8

CXCL8 was originally discovered and purified independently by a number of laboratories as a neutrophil chemotactic and activating factor. It was also referred to as neutrophil chemotactic factor (NCF), neutrophil activating protein (NAP), monocyte-derived neutrophil chemotactic factor (MDNCF), T-lymphocyte chemotactic factor (TCF), granulocyte chemotactic protein (GCP) and leukocyte adhesion inhibitor (LAI). Many cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, chondrocytes, and various tumor cell lines, can produce CXCL8 in response to a wide variety of
pro-inflammatory stimuli such as exposure to IL-1, TNF, LPS, and viruses. CXCL8 is a member of the alpha (CXC) subfamily of chemokines, which also includes platelet factor 4, GRO, IP-10, etc.

CXCL8 is a potent chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil specific granules and azurophilic granules. CXCL8 induces expression of the cell adhesion molecules CD11/CD18 and enhances the adherence of neutrophils to endothelial cells and sub-endothelial matrix proteins. Besides neutrophils, CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has been reported to be a co-mitogen for keratinocytes and was also shown to be an autocrine growth factor for melanoma cells. CXCL8 was also reported to be angiogenic both in vivo and in vitro.

  • Long Name:
    Interleukin 8
  • Entrez Gene IDs:
    3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
  • Alternate Names:
    3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
    Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
    Endocrinology, 2015;156(2):670-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA detection
  2. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  3. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011;186(7):4110-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Electrochemiluminescence
  4. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  5. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Type: Urine
    Application: Antibody Array Development
  6. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  7. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008;43(2):187-93.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  8. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008;181(1):557-65.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Electrochemiluminescence
  9. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007;13(19):5719-28.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: ELISA Development
  10. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  11. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Type: Plasma
    Application: ELISA Development
  12. Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
    Authors: Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, Machugh DE, Baird AW
    Vet. Immunol. Immunopathol., 2007;116(1):59-68.
    Species: Bovine
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  13. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Type: Serum
    Application: Luminex Development
  14. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  15. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: Human
    Sample Type: Follicular Fluid
    Application: ELISA Development
  16. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  17. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  18. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  19. PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
    Authors: Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
    Am. J. Obstet. Gynecol., 2006;195(1):103-8.
    Species: Human
    Sample Type: Peritoneal Fluid
    Application: ELISA Development
  20. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
    Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
    Acta Obstet Gynecol Scand, 2005;84(6):551-7.
    Species: Human
    Sample Type: Amniotic Fluid
    Application: ELISA Development
  21. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: Array Development
  22. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  23. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  24. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  25. Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
    Authors: Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
    J. Immunol., 2002;169(11):6515-21.
    Species: Human
    Sample Type: BALF
    Application: ELISA Development
  26. Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
    Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
    Am. J. Kidney Dis., 2002;39(5):1078-87.
    Species: Human
    Sample Type: Whole Cells
    Application: ELISA Development
  27. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001;14(6):705-14.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  28. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000;47(5):592-7.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
  29. Peritonitis in the baboon: a primate model which stimulates human sepsis.
    Authors: Kinasewitz GT, Chang AC, Peer GT, Hinshaw LB, Taylor FB
    Shock, 2000;13(2):100-9.
    Species: Primate- Papio cynocephalus (Baboon)
    Sample Type: Plasma
    Application: ELISA Development
Expand to show all 29 Citations
Isotype Controls
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Normal Goat IgG Biotinylated Control

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