Human IL-8/CXCL8 Antibody

Catalog # Availability Size / Price Qty
MAB208-SP
MAB208-100
MAB208-500
Best Seller
Detection of Human IL-8/CXCL8 by Western Blot.
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Product Details
Citations (119)
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Reviews (10)

Human IL-8/CXCL8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 6217
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
3 µg/mL
THP‑1 human acute monocytic leukemia cell line treated (+) with PMA and LPS
Simple Western
50 µg/mL
Human PBMC conditioned media treated with LPS
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Human IL-8/CXCL8 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL-8/CXCL8 Biotinylated Antibody (Catalog # BAF208)

Standard: Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL)

Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human IL-8/CXCL8 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA and 10 ug/mL LPS for 24 hours and 3 hours. PVDF membrane was probed with 3 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IL-8/CXCL8 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry IL-8/CXCL8 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western Detection of Human IL-8/CXCL8 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human IL-8/CXCL8 by Simple WesternTM. Simple Western lane view shows human PBMC conditioned media untreated (-) or treated (+) with 1 μg/mL LPS for 24 hrs, loaded at 0.2 mg/mL. A specific band was detected for IL-8/CXCL8 at approximately 8 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Neutralization Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. View Larger

Chemotaxis Induced by IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically 0.08-0.4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

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Citations for Human IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

119 Citations: Showing 1 - 10
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  1. Catecholamines Promote Ovarian Cancer Progression through Secretion of CXC-Chemokines
    Authors: Kim, HJ;Chang, HK;Lee, YM;Heo, K;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Oleic acid-induced metastasis of KRAS/p53-mutant colorectal cancer relies on concurrent KRAS activation and IL-8 expression bypassing EGFR activation
    Authors: Shen, CJ;Chan, RH;Lin, BW;Li, NC;Huang, YH;Chang, WC;Chen, BK;
    Theranostics
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Suppression of TGF beta -Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38-NF-kB Pathway in Carcinoma Cells
    Authors: Yang, JL;Lin, WL;Tai, SB;Ciou, YS;Chung, CL;Chen, JJ;Liu, PF;Lin, MW;Chen, CL;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. High FOXA1 levels induce ER transcriptional reprogramming, a pro-metastatic secretome, and metastasis in endocrine-resistant breast cancer
    Authors: Fu, X;Pereira, R;Liu, CC;De Angelis, C;Shea, MJ;Nanda, S;Qin, L;Mitchell, T;Cataldo, ML;Veeraraghavan, J;Sethunath, V;Giuliano, M;Gutierrez, C;Győrffy, B;Trivedi, MV;Cohen, O;Wagle, N;Nardone, A;Jeselsohn, R;Rimawi, MF;Osborne, CK;Schiff, R;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. IL8 derived from macrophages inhibits CD8+ T-cell function by downregulating TIM3 expression through IL8-CXCR2 axis in patients with advanced colorectal cancer
    Authors: Zhao, C;Wang, D;Li, Z;Zhang, Z;Xu, Y;Liu, J;Lei, Q;Han, D;Huo, Y;Liu, S;Li, L;Zhang, Y;
    International immunopharmacology
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC, Neutralization
  6. Discovery of senolytics using machine learning
    Authors: Smer-Barreto, V;Quintanilla, A;Elliott, RJR;Dawson, JC;Sun, J;Campa, VM;Lorente-Macías, Á;Unciti-Broceta, A;Carragher, NO;Acosta, JC;Oyarzún, DA;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPAR?-CPT1A signaling to potentiate lymphatic metastasis
    Authors: Huang, J;Wang, X;Wen, J;Zhao, X;Wu, C;Wang, L;Cao, X;Dong, H;Xu, X;Huang, F;Zhu, W;Wang, M;
    Cancer cell international
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. RanBP1: A Potential Therapeutic Target for Cancer Stem Cells in Lung Cancer and Glioma
    Authors: YJ Kahm, IG Kim, RK Kim
    International Journal of Molecular Sciences, 2023-04-06;24(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Effect of teriparatide on drug treatment of tuberculous spondylitis: an experimental study
    Authors: S Lee, YJ Seo, JY Choi, X Che, HJ Kim, SY Eum, MS Hong, SK Lee, DC Cho
    Scientific Reports, 2022-12-15;12(1):21667.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  10. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype
    Authors: W Zeng, L Xiong, W Wu, S Li, J Liu, L Yang, L Lao, P Huang, M Zhang, H Chen, N Miao, Z Lin, Z Liu, X Yang, J Wang, P Wang, E Song, Y Yao, Y Nie, J Chen, D Huang
    Oncogene, 2022-11-22;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Modeling human HSV infection via a vascularized immune-competent skin-on-chip platform
    Authors: S Sun, L Jin, Y Zheng, J Zhu
    Nature Communications, 2022-09-19;13(1):5481.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Neutralization
  12. The AGEs/RAGE Transduction Signaling Prompts IL-8/CXCR1/2-Mediated Interaction between Cancer-Associated Fibroblasts (CAFs) and Breast Cancer Cells
    Authors: MF Santolla, M Talia, F Cirillo, D Scordamagl, S De Rosis, A Spinelli, AM Miglietta, B Nardo, G Filippelli, EM De Frances, A Belfiore, R Lappano, M Maggiolini
    Cells, 2022-08-04;11(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Neuropilin-2 axis in regulating secretory phenotype of neuroendocrine-like prostate cancer cells and its implication in therapy resistance
    Authors: R Islam, J Mishra, NS Polavaram, S Bhattachar, Z Hong, S Bodas, S Sharma, A Bouska, T Gilbreath, AM Said, LM Smith, BA Teply, MH Muders, SK Batra, K Datta, S Dutta
    Oncogene, 2022-07-19;40(3):111097.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
    Authors: T Walle, JA Kraske, B Liao, B Lenoir, C Timke, E von Bohlen, F Tran, P Griebel, D Albrecht, A Ahmed, M Suarez-Car, A Jiménez-Sá, T Beikert, A Tietz-Dahl, AN Menevse, G Schmidt, M Brom, JHW Pahl, W Antonopoul, M Miller, RL Perez, F Bestvater, NA Giese, P Beckhove, P Rosenstiel, D Jäger, O Strobel, D Pe'er, N Halama, J Debus, A Cerwenka, PE Huber
    Science Advances, 2022-03-23;8(12):eabh4050.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Flow cytometric single cell-based assay to simultaneously detect cell death, cell cycling, DNA content and cell senescence
    Authors: E Lieschke, Z Wang, C Chang, CE Weeden, GL Kelly, A Strasser
    Cell Death and Differentiation, 2022-03-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Occludin facilitates tumour angiogenesis in bladder cancer by regulating IL8/STAT3 through STAT4
    Authors: F Yang, XQ Liu, JZ He, SP Xian, PF Yang, ZY Mai, M Li, Y Liu, XD Zhang
    Journal of Cellular and Molecular Medicine, 2022-02-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Tumor-Associated Microglia/Macrophages as a Predictor for Survival in Glioblastoma and Temozolomide-Induced Changes in CXCR2 Signaling with New Resistance Overcoming Strategy by Combination Therapy
    Authors: RM Urbantat, C Jelgersma, S Brandenbur, M Nieminen-K, I Kremenetsk, J Zollfrank, S Mueller, K Rubarth, A Koch, P Vajkoczy, G Acker
    International Journal of Molecular Sciences, 2021-10-16;22(20):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  18. Extracellular Hsp90alpha Promotes Tumor Lymphangiogenesis and Lymph Node Metastasis in Breast Cancer
    Authors: Q Hou, S Chen, Q An, B Li, Y Fu, Y Luo
    International Journal of Molecular Sciences, 2021-07-20;22(14):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: Co-Immunoprecipitation, ICC, IHC, Western Blot
  19. COX2 regulates senescence secretome composition and senescence surveillance through PGE2
    Authors: S Gonçalves, K Yin, Y Ito, A Chan, I Olan, S Gough, L Cassidy, E Serrao, S Smith, A Young, M Narita, M Hoare
    Cell Reports, 2021-03-16;34(11):108860.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  20. Caspase-1 has a critical role in blood-brain barrier injury and its inhibition contributes to multifaceted repair
    Authors: H Israelov, O Ravid, D Atrakchi, D Rand, S Elhaik, Y Bresler, R Twitto-Gre, L Omesi, S Liraz-Zalt, F Gosselet, M Schnaider, I Cooper
    J Neuroinflammation, 2020-09-09;17(1):267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Chemotherapy-induced IL-8 upregulates MDR1/ABCB1 in tumor blood vessels and results in unfavorable outcome
    Authors: H Kikuchi, N Maishi, DA Annan, M Towfik Ala, R Ibrahim Ha, M Sato, M Morimoto, R Takeda, K Ishizuka, R Matsumoto, T Akino, K Tsuchiya, T Abe, T Osawa, N Miyajima, S Maruyama, T Harabayash, M Azuma, K Yamashiro, K Ameda, A Kashiwagi, Y Matsuno, Y Hida, N Shinohara, K Hida
    Cancer Res., 2020-06-14;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: IHC, Neutralization
  22. Primary tumors release ITGBL1-rich extracellular vesicles to promote distal metastatic tumor growth through fibroblast-niche formation
    Authors: Q Ji, L Zhou, H Sui, L Yang, X Wu, Q Song, R Jia, R Li, J Sun, Z Wang, N Liu, Y Feng, X Sun, G Cai, Y Feng, J Cai, Y Cao, G Cai, Y Wang, Q Li
    Nat Commun, 2020-03-05;11(1):1211.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Neutrophil Activities in Human Ocular Toxoplasmosis: An In Vitro Study With Human Cells
    Authors: LM Ashander, S Lie, Y Ma, E Rochet, JM Washington, JM Furtado, B Appukuttan, JR Smith
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4652-4660.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  24. Microenvironmental IL1beta promotes breast cancer metastatic colonisation in the bone via activation of Wnt signalling
    Authors: R Eyre, DG Alférez, A Santiago-G, K Spence, JC McConnell, C Hart, BM Simões, D Lefley, C Tulotta, J Storer, A Gurney, N Clarke, M Brown, SJ Howell, AH Sims, G Farnie, PD Ottewell, RB Clarke
    Nat Commun, 2019-11-01;10(1):5016.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  25. Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
    Authors: A Schulz, L Jiang, L de Vor, M Ehrström, F Wermeling, L Eidsmo, K Melican
    Cell Rep, 2019-10-29;29(5):1074-1081.e5.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Blocking
  26. TCR- or Cytokine-Activated CD8+ Mucosal-Associated Invariant T Cells Are Rapid Polyfunctional Effectors That Can Coordinate Immune Responses
    Authors: R Lamichhane, M Schneider, SM de la Harp, TWR Harrop, RF Hannaway, PK Dearden, JR Kirman, JDA Tyndall, AJ Vernall, JE Ussher
    Cell Rep, 2019-09-17;28(12):3061-3076.e5.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  27. Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3
    Authors: M Borghesan, J Fafián-Lab, O Eleftheria, P Carpintero, M Paez-Ribes, G Vizcay-Bar, A Swisa, D Kolodkin-G, P Ximénez-Em, R Lowe, B Martín-Mar, H Peinado, J Muñoz, RA Fleck, Y Dor, I Ben-Porath, A Vossenkamp, D Muñoz-Espi, A O'Loghlen
    Cell Rep, 2019-06-25;27(13):3956-3971.e6.
    Species: Human
    Sample Types: Cell Lysates, Extracellular Vesicles
    Applications: ICC, Western Blot
  28. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019-06-11;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  29. The Hepatic Microenvironment and TRAIL-R2 Impact Outgrowth of Liver Metastases in Pancreatic Cancer after Surgical Resection
    Authors: L Miarka, C Hauser, O Helm, D Holdhof, S Beckinger, JH Egberts, JP Gundlach, L Lenk, S Rahn, W Mikulits, A Trauzold, S Sebens
    Cancers (Basel), 2019-05-29;11(6):.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Neutralization
  30. Differentiated fibrocytes assume a functional mesenchymal phenotype with regenerative potential
    Authors: C Ling, K Nishimoto, Z Rolfs, LM Smith, BL Frey, NV Welham
    Sci Adv, 2019-05-08;5(5):eaav7384.
    Species: Rat
    Sample Types: In Vivo
    Applications: Bioassay
  31. Circulating Truncated Alpha-1 Antitrypsin Glycoprotein in Patient Plasma Retains Anti-Inflammatory Capacity
    Authors: EP Reeves, DM Dunlea, K McQuillan, CA O'Dwyer, TP Carroll, R Saldova, PR Akepati, MR Wormald, OJ McElvaney, V Shutchaida, M Henry, P Meleady, J Keenan, DC Liberti, DN Kotton, PM Rudd, AA Wilson, NG McElvaney
    J. Immunol., 2019-02-22;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Binding Assay
  32. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019-02-06;22(0):101116.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  33. Varicella Zoster Virus Induces Differential Cell-Type Specific Responses in Human Corneal Epithelial Cells and Keratocytes
    Authors: CN Como, AN Bubak, AM Blackmon, D Jones, NH Mueller, R Davidson, MA Nagel
    Invest. Ophthalmol. Vis. Sci., 2019-02-01;60(2):704-711.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. L-selectin expression is regulated by CXCL8-induced reactive oxygen species produced during human neutrophil rolling
    Authors: MJ Domínguez-, E Armas-Gonz, A Herrera-Ga, M Arce-Franc, M Feria, M Vicente-Ma, A Martínez-R, F Sánchez-Ma, F Díaz-Gonzá
    Eur. J. Immunol., 2018-12-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  35. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018-08-15;9(1):3267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  36. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
    Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
    Psychoneuroendocrinology, 2018-07-13;9(1):2705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  37. ShRNA-mediated knock-down of CXCL8 inhibits tumor growth in colorectal liver metastasis
    Authors: A Kumar, M Cherukumil, SH Mahmoudpou, K Brand, OR Bandapalli
    Biochem. Biophys. Res. Commun., 2018-04-23;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis
    Authors: Q Liu, A Li, S Yu, S Qin, N Han, RG Pestell, X Han, K Wu
    J Hematol Oncol, 2018-04-10;11(1):53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  39. Age-Related Changes to Human Tear Composition
    Authors: A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini
    Invest. Ophthalmol. Vis. Sci., 2018-04-01;59(5):2024-2031.
    Species: Human
    Sample Types: Tears
    Applications: Western Blot
  40. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner
    Authors: F Melo-Gonza, TM Fenton, C Forss, C Smedley, A Goenka, AS MacDonald, DJ Thornton, MA Travis
    J. Biol. Chem., 2018-03-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  41. Galectin-3 is an amplifier of the interleukin-1?-mediated inflammatory response in corneal keratinocytes
    Authors: Y Uchino, AM Woodward, J Mauris, K Peterson, P Verma, UJ Nilsson, J Rajaiya, P Argüeso
    Immunology, 2018-02-15;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  42. HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome
    Authors: S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce
    Oncogene, 2018-01-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Inhibition
  43. Endothelial cell leptin receptors, leptin and interleukin-8 in the pathogenesis of preeclampsia: An in-vitro study
    Authors: S Arl?er
    Turk J Obstet Gynecol, 2017-12-30;14(4):220-227.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  44. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
    Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
    PLoS ONE, 2017-09-07;12(9):e0184039.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  45. Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease
    Authors: MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves
    EBioMedicine, 2017-08-16;23(0):173-184.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  46. Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis
    Authors: Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng
    Stem Cells, 2017-06-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  47. Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells
    Authors: B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas
    Sci Rep, 2017-05-24;7(1):2329.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  48. Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling
    Authors: HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim
    PLoS ONE, 2017-04-13;12(4):e0175159.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Neutralization
  49. Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells
    Authors: J Liu, G Song, X Lin, X Pang, T Meng
    Exp Ther Med, 2017-02-21;13(4):1447-1455.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  50. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017-02-16;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  51. Histone Deacetylase (HDAC) Inhibition Induces I?B Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells
    Authors: HR Gatla, Y Zou, MM Uddin, B Singha, P Bu, A Vancura, I Vancurova
    J. Biol. Chem., 2017-02-06;292(12):5043-5054.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  52. Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells
    Authors: Yuh-Lin Wu
    Innate Immun, 2016-11-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
    Cancer Res., 2016-11-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells
    Int J Mol Sci, 2016-08-19;17(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  55. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016-08-15;18(9):979-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  56. Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells
    Sci Rep, 2016-08-10;6(0):31087.
    Species: Bacteria - E. Coli
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  57. The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer
    Authors: Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang
    PLoS ONE, 2016-04-14;11(4):e0152789.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  58. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
    Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
    Stem Cells, 2016-01-13;34(4):1112-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  59. Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop.
    Authors: Chen L, Min L, Wang X, Zhao J, Chen H, Qin J, Chen W, Shen Z, Tang Z, Gan Q, Ruan Y, Sun Y, Qin X, Gu J
    Cancer Res, 2015-07-21;75(18):3832-41.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  60. Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
    Authors: Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S
    Cancer Res, 2015-05-07;75(13):2629-40.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  61. Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.
    Authors: Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J
    Eur J Immunol, 2014-12-01;45(1):101-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  62. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
    Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
    Endocrinology, 2014-11-26;156(2):670-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  63. The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis.
    Authors: McElvaney O, O'Reilly N, White M, Lacey N, Pohl K, Gerlza T, Bergin D, Kerr H, McCarthy C, O'Brien M, Adage T, Kungl A, Reeves E, McElvaney N
    Mol Immunol, 2014-11-02;63(2):550-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
    Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
    J Immunol, 2014-05-28;193(1):234-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  65. Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
    Authors: Moelants, Eva A V, Loozen, Gitte, Mortier, Anneleen, Martens, Erik, Opdenakker, Ghislain, Mizgalska, Danuta, Szmigielski, Borys, Potempa, Jan, Van Damme, Jo, Teughels, Wim, Proost, Paul
    Infect Immun, 2014-03-31;82(6):2511-9.
    Species: Bacteria - Porphyromonas gingivalis
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  66. Chemokine CXCL8 promotes HIV-1 replication in human monocyte-derived macrophages and primary microglia via nuclear factor-kappaB pathway.
    Authors: Mamik M, Ghorpade A
    PLoS ONE, 2014-03-24;9(3):e92145.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  67. Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
    Authors: Windheim M, Hansen B
    Cell Signal, 2013-09-28;26(1):49-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  68. CD82/KAI expression prevents IL-8-mediated endothelial gap formation in late-stage melanomas.
    Authors: Khanna, P, Chung, C-Y, Neves, R I, Robertson, G P, Dong, C
    Oncogene, 2013-07-22;33(22):2898-908.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: ELISA Development (Capture), Western Blot
  69. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012-11-23;26(3):357-69.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  70. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012-09-17;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  71. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
    Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
    Blood, 2012-09-13;120(18):3793-802.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  72. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012-07-03;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  73. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
    Authors: Meda C, Molla F, De Pizzol M
    J. Immunol., 2012-03-21;188(8):4081-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  74. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012-02-15;7(2):e31646.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  75. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 2011-12-21;5(2):140-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  76. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011-02-23;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  77. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  78. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010-10-22;185(11):7047-56.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  79. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010-03-24;184(0):5263-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  80. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2009-12-21;184(3):1642-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  81. Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion.
    Authors: Rodier F, Coppe JP, Patil CK, Hoeijmakers WA, Munoz DP, Raza SR, Freund A, Campeau E, Davalos AR, Campisi J
    Nat. Cell Biol., 2009-07-13;11(8):973-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009-06-27;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  83. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009-06-12;297(2):L388-400.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  84. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008-07-17;43(2):187-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  85. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008-07-01;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  86. IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
    Authors: Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG
    J. Immunol., 2008-07-01;181(1):669-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  87. Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
    Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
    Invest. Ophthalmol. Vis. Sci., 2008-06-14;49(10):4578-89.
    Species: Human, Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  88. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007-12-01;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  89. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007-11-01;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  90. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007-10-22;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  91. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007-10-01;13(19):5719-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  92. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007-09-21;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  93. Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
    Authors: Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, Machugh DE, Baird AW
    Vet. Immunol. Immunopathol., 2007-01-09;116(1):59-68.
    Species: Bovine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  94. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006-11-15;119(10):2313-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  95. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2006-10-04;192(1):56-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  96. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006-09-18;74(12):6730-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  97. Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice.
    Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K
    Cancer Res., 2006-07-01;66(13):6699-707.
    Species: Complex Species Category
    Sample Types:
    Applications: Neutralization
  98. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Blister Fluid, Cell Culture Supernates
    Applications: ELISA Development, Neutralization
  99. Circulating CD34+ progenitor cells modulate host angiogenesis and inflammation in vivo.
    Authors: Popa ER, Harmsen MC, Tio RA, van der Strate BW, Brouwer LA, Schipper M, Koerts J, De Jongste MJ, Hazenberg A, Hendriks M, van Luyn MJ
    J. Mol. Cell. Cardiol., 2006-06-14;41(1):86-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  100. PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
    Authors: Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
    Am. J. Obstet. Gynecol., 2006-04-25;195(1):103-8.
    Species: Human
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  101. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  102. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006-01-19;34(5):616-24.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  103. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
    Applications: ELISA Development
  104. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  105. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005-06-01;46(6):659-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  106. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
    Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
    Acta Obstet Gynecol Scand, 2005-06-01;84(6):551-7.
    Species: Human
    Sample Types: Amniotic Fluid
    Applications: ELISA Development
  107. The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium.
    Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
    J. Neuroimmunol., 2005-04-01;161(1):49-60.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: ICC
  108. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
    Authors: Penninckx F, Ectors N
    Exp. Cell Res., 2005-02-15;303(2):331-42.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  109. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004-06-01;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  110. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004-05-13;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  111. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004-01-01;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  112. ELR+ CXC chemokines in human milk.
    Authors: Maheshwari A, Christensen RD, Calhoun DA
    Cytokine, 2003-11-07;24(3):91-102.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  113. Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
    Authors: Park JS, 107290, Kim YS, Jee YK, Myong NH, Lee KY
    Active immunotherapy against pathogenic Cis pT231-tau suppresses neurodegeneration in traumatic brain injury mouse models, 2003-05-01;57(5):463-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  114. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002-11-01;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  115. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002-06-15;168(12):6244-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  116. Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
    Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
    Am. J. Kidney Dis., 2002-05-01;39(5):1078-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  117. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002-04-15;165(8):1161-71.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  118. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001-06-01;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  119. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000-05-01;47(5):592-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

FAQs

  1. Are mouse or rat CXCL8/IL-8 products offered?

    • Mouse and rat do not have a gene which encodes CXCL8/IL-8. In mouse, the functional homologs to human CXCL8/IL-8 are CXCL1/KC and CXCL2/MIP-2. In rat, the functional homolog to CXCL8/human IL-8 is CXCL3/CINC-2.

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Human IL-8/CXCL8 Antibody
By Anonymous on 08/13/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Colon cancer tissue Species: Human

IF of CXCL8 was done using dilution of 1:2000 in 2.5% horse serum/PBS and incubated at 4 degree overnight, and AF488 conjugated secondary antibody was used for 1h at RT.


Human IL-8/CXCL8 Antibody
By Anonymous on 08/06/2021
Application: IHC Sample Tested: Brain tumor Species: Human

Yellow arrows on the picture indicate staining in tumor cells.


Human IL-8/CXCL8 Antibody
By Anonymous on 07/30/2021
Application: WB Sample Tested: macrophage Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 03/16/2020
Application: Func Sample Tested: Whole Cells Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 05/27/2019
Application: B/N Sample Tested: Breast cancer cells,Serum-free Cell Culture Media Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human IL-8/CXCL8 Antibody
By Zoia Levashova on 11/01/2018
Application: MSD assay Sample Tested: Vitreous humor Species: Cynomolgus Monkey and Porcine

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC).
A: Paired with SulfoTag-modified AF-208-NA as a detection antibody. Calibration curves with Recombinant Human IL-8 (208-IL-010/CF) is shown (dynamic range 1.6-25,000 pg/ml).
B: Paired with SulfoTag-modified MAB-535 to detect porcine IL-8. Calibration curves with Recombinant Porcine IL-8 (535-IN/CF) is shown (dynamic range 1.6-25,000 pg/ml).


Human IL-8/CXCL8 Antibody
By Prasanna Ekambaram on 05/11/2018
Application: B/N Sample Tested: Breast cancer cells Species: Human

We used this IL8 neutralizing antibody (600 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize IL8 present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II.
CM (+/- IL8 Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay.
Cancer Res; 78(5) March 1, 2018 (Fig 6F)


Human IL-8/CXCL8 Antibody
By Leslie Priddy on 04/03/2018
Application: ELISA Sample Tested: Serum Species: Human

Human IL-8/CXCL8 Antibody
By Michelle Chen on 04/26/2017
Application: B/N Sample Tested: Neutrophils Species: Human