Human IL-8/CXCL8 Antibody

Catalog # Availability Size / Price Qty
MAB208-SP
MAB208-100
MAB208-500
Chemotaxis Induced byIL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody.
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Product Details
Citations (81)
FAQs
Supplemental Products
Reviews (5)

Human IL-8/CXCL8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-8/CXCL8 in ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant porcine IL-8/CXCL8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 alpha is observed.
Source
Monoclonal Mouse IgG1 Clone # 6217
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-8/CXCL8
Ser28-Ser99
Accession # P10145
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Human IL-8/CXCL8 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IL-8/CXCL8 Biotinylated Antibody (Catalog # BAF208)

Standard: Recombinant Human IL-8/CXCL8 Protein (Catalog # 208-IL)

Neutralization
Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.08-0.4 µg/mL in the presence of 20 ng/mL Recombinant Human IL-8/CXCL8.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Neutralization Chemotaxis Induced byIL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody. View Larger

Chemotaxis Induced by
IL-8/CXCL8 and Neutralization by Human IL-8/CXCL8 Antibody.
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically
0.08-0.4 µg/mL.

Immunocytochemistry IL-8/CXCL8 in Human PBMCs. View Larger

IL-8/CXCL8 in Human PBMCs. IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-8/CXCL8

CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.

Long Name
Interleukin 8
Entrez Gene IDs
3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
Alternate Names
3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1

Product Datasheets

Citations for Human IL-8/CXCL8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

81 Citations: Showing 1 - 10
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  1. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018;9(1):3267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
    Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
    Psychoneuroendocrinology, 2018;9(1):2705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. DACH1 antagonizes CXCL8 to repress tumorigenesis of lung adenocarcinoma and improve prognosis
    Authors: Q Liu, A Li, S Yu, S Qin, N Han, RG Pestell, X Han, K Wu
    J Hematol Oncol, 2018;11(1):53.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Intestinal mucin activates human dendritic cells and IL-8 production in a glycan-specific manner
    Authors: F Melo-Gonza, TM Fenton, C Forss, C Smedley, A Goenka, AS MacDonald, DJ Thornton, MA Travis
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  5. HSP90 inhibition alters the chemotherapy-driven rearrangement of the oncogenic secretome
    Authors: S di Martino, CA Amoreo, B Nuvoli, R Galati, S Strano, F Facciolo, G Alessandri, HI Pass, G Ciliberto, G Blandino, R De Maria, M Cioce
    Oncogene, 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: inhibition
  6. Age-Related Changes to Human Tear Composition
    Authors: A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini
    Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031.
    Species: Human
    Sample Types: Tear Fluid
    Applications: WB
  7. Human adipose-derived mesenchymal stem cell-secreted CXCL1 and CXCL8 facilitate breast tumor growth by promoting angiogenesis
    Authors: Y Wang, J Liu, Q Jiang, J Deng, F Xu, X Chen, F Cheng, Y Zhang, Y Yao, Z Xia, X Xu, X Su, M Huang, L Dai, Y Yang, S Zhang, D Yu, RC Zhao, Y Wei, H Deng
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  8. Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease
    Authors: J Ikari, AJ Nelson, J Obaid, A Giron-Mart, K Ikari, F Makino, S Iwasawa, Y Gunji, M Farid, X Wang, H Basma, D Demeo, C Feghali-Bo, O Holz, K Rabe, X Liu, SI Rennard
    PLoS ONE, 2017;12(9):e0184039.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  9. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Disruption of outer blood-retinal barrier by Toxoplasma gondii-infected monocytes is mediated by paracrinely activated FAK signaling
    Authors: HB Song, HO Jun, JH Kim, YH Lee, MH Choi, JH Kim
    PLoS ONE, 2017;12(4):e0175159.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Neut
  11. Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells
    Authors: B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas
    Sci Rep, 2017;7(1):2329.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  12. Upregulated unique long 16 binding protein 1 detected in preeclamptic placenta affects human extravillous trophoblast cell line (HTR-8/SVneo) invasion by modulating the function of uterine natural killer cells
    Authors: J Liu, G Song, X Lin, X Pang, T Meng
    Exp Ther Med, 2017;13(4):1447-1455.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  13. Neutrophil Membrane Cholesterol Content is a Key Factor in Cystic Fibrosis Lung Disease
    Authors: MM White, P Geraghty, E Hayes, S Cox, W Leitch, B Alfawaz, GM Lavelle, OJ McElvaney, R Flannery, J Keenan, P Meleady, M Henry, M Clynes, C Gunaratnam, NG McElvaney, EP Reeves
    EBioMedicine, 2017;23(0):173-184.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  14. Histone Deacetylase (HDAC) Inhibition Induces I?B Kinase (IKK)-dependent Interleukin-8/CXCL8 Expression in Ovarian Cancer Cells
    Authors: HR Gatla, Y Zou, MM Uddin, B Singha, P Bu, A Vancura, I Vancurova
    J. Biol. Chem., 2017;292(12):5043-5054.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  15. The Proton-Sensing G-Protein Coupled Receptor GPR4 Promotes Angiogenesis in Head and Neck Cancer
    Authors: Z Jing, H Xu, X Chen, Q Zhong, J Huang, Y Zhang, W Guo, Z Yang, S Ding, P Chen, Z Huang
    PLoS ONE, 2016;11(4):e0152789.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  16. Growth Hormone Releasing Peptide-2 Attenuation of Protein Kinase C-Induced Inflammation in Human Ovarian Granulosa Cells
    Int J Mol Sci, 2016;17(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  17. Tsr Chemoreceptor Interacts With IL-8 Provoking E. coli Transmigration Across Human Lung Epithelial Cells
    Sci Rep, 2016;6(0):31087.
    Species: E. coli
    Sample Types: Cell Lysates
    Applications: IP
  18. NOTCH1 mediates a switch between two distinct secretomes during senescence
    Authors: M Hoare, Y Ito, TW Kang, MP Weekes, NJ Matheson, DA Patten, S Shetty, AJ Parry, S Menon, R Salama, R Antrobus, K Tomimatsu, W Howat, PJ Lehner, L Zender, M Narita
    Nat. Cell Biol, 2016;18(9):979-92.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  19. Novel augmentation by bufalin of protein kinase C-induced cyclooxygenase-2 and IL-8 production in human breast cancer cells
    Authors: Yuh-Lin Wu
    Innate Immun, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Human pancreatic cancer cells induce a MyD88-dependent stromal response to promote a tumor-tolerant immune microenvironment
    Cancer Res., 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
    Authors: Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S
    Cancer Res, 2015;75(13):2629-40.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neut
  22. Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines.
    Authors: Gouwy M, De Buck M, Portner N, Opdenakker G, Proost P, Struyf S, Van Damme J
    Eur J Immunol, 2015;45(1):101-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  23. The effect of the decoy molecule PA401 on CXCL8 levels in bronchoalveolar lavage fluid of patients with cystic fibrosis.
    Authors: McElvaney O, O'Reilly N, White M, Lacey N, Pohl K, Gerlza T, Bergin D, Kerr H, McCarthy C, O'Brien M, Adage T, Kungl A, Reeves E, McElvaney N
    Mol Immunol, 2015;63(2):550-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Loss of RACK1 Promotes Metastasis of Gastric Cancer by Inducing a miR-302c/IL8 Signaling Loop.
    Authors: Chen L, Min L, Wang X, Zhao J, Chen H, Qin J, Chen W, Shen Z, Tang Z, Gan Q, Ruan Y, Sun Y, Qin X, Gu J
    Cancer Res, 2015;75(18):3832-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  25. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
    Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
    Endocrinology, 2015;156(2):670-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  26. Interleukin-1-induced activation of the small GTPase Rac1 depends on receptor internalization and regulates gene expression.
    Authors: Windheim M, Hansen B
    Cell Signal, 2014;26(1):49-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  27. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
    Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
    J Immunol, 2014;193(1):234-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  28. Citrullination and proteolytic processing of chemokines by Porphyromonas gingivalis.
    Authors: Moelants, Eva A V, Loozen, Gitte, Mortier, Anneleen, Martens, Erik, Opdenakker, Ghislain, Mizgalska, Danuta, Szmigielski, Borys, Potempa, Jan, Van Damme, Jo, Teughels, Wim, Proost, Paul
    Infect Immun, 2014;82(6):2511-9.
    Species: Bacteria - Porphyromonas gingivalis
    Sample Types: Cell Culture Supernates
    Applications: ELISA detection
  29. CD82/KAI expression prevents IL-8-mediated endothelial gap formation in late-stage melanomas.
    Authors: Khanna, P, Chung, C-Y, Neves, R I, Robertson, G P, Dong, C
    Oncogene, 2013;33(22):2898-908.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  30. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  31. Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
    J. Exp. Med., 2012;209(10):1813-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  32. Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam.
    Authors: Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T
    Mod Pathol, 2012;26(3):357-69.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  33. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  34. Neutralization of the IL-17 axis diminishes neutrophil invasion and protects from ischemic stroke.
    Authors: Gelderblom M, Weymar A, Bernreuther C, Velden J, Arunachalam P, Steinbach K, Orthey E, Arumugam T, Leypoldt F, Simova O, Thom V, Friese M, Prinz I, Holscher C, Glatzel M, Korn T, Gerloff C, Tolosa E, Magnus T
    Blood, 2012;120(18):3793-802.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  35. Semaphorin 4A exerts a proangiogenic effect by enhancing vascular endothelial growth factor-A expression in macrophages.
    Authors: Meda C, Molla F, De Pizzol M
    J. Immunol., 2012;188(8):4081-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  36. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  37. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescence
  38. Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis.
    Authors: Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F
    J. Immunol., 2010;184(0):5263-70.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  39. Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
    Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
    J. Immunol., 2010;185(11):7047-56.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  40. IL-8 dictates glycosaminoglycan binding and stability of IL-18 in cystic fibrosis.
    Authors: Reeves EP, Williamson M, Byrne B, Bergin DA, Smith SG, Greally P, O'Kennedy R, O'Neill SJ, McElvaney NG
    J. Immunol., 2010;184(3):1642-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  41. Persistent DNA damage signalling triggers senescence-associated inflammatory cytokine secretion.
    Authors: Rodier F, Coppe JP, Patil CK, Hoeijmakers WA, Munoz DP, Raza SR, Freund A, Campeau E, Davalos AR, Campisi J
    Nat. Cell Biol., 2009;11(8):973-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  42. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  43. Oxidized alpha1-antitrypsin stimulates the release of monocyte chemotactic protein-1 from lung epithelial cells: potential role in emphysema.
    Authors: Li Z, Alam S, Wang J, Sandstrom CS, Janciauskiene S, Mahadeva R
    Am. J. Physiol. Lung Cell Mol. Physiol., 2009;297(2):L388-400.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  44. IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis.
    Authors: Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG
    J. Immunol., 2008;181(1):669-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  45. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescence
  46. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008;43(2):187-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  47. Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
    Authors: Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
    Invest. Ophthalmol. Vis. Sci., 2008;49(10):4578-89.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  48. Human epithelial cells establish direct antifungal defense through TLR4-mediated signaling.
    Authors: Weindl G, Naglik JR, Kaesler S, Biedermann T, Hube B, Korting HC, Schaller M
    J. Clin. Invest., 2007;117(12):3664-3672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  49. Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells.
    Authors: Fitzgerald DC, Meade KG, McEvoy AN, Lillis L, Murphy EP, Machugh DE, Baird AW
    Vet. Immunol. Immunopathol., 2007;116(1):59-68.
    Species: Bovine
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  50. Eotaxin-2 and colorectal cancer: a potential target for immune therapy.
    Authors: Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE
    Clin. Cancer Res., 2007;13(19):5719-28.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  51. L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion.
    Authors: Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM
    Atherosclerosis, 2007;192(1):56-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  52. Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
    Authors: Pfleger C, Schloot N, ter Veld F
    J. Immunol. Methods, 2007;329(1):214-8.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development
  53. Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines.
    Authors: Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA
    Clin. Chem., 2007;53(11):2010-2.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  54. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  55. Circulating CD34+ progenitor cells modulate host angiogenesis and inflammation in vivo.
    Authors: Popa ER, Harmsen MC, Tio RA, van der Strate BW, Brouwer LA, Schipper M, Koerts J, De Jongste MJ, Hazenberg A, Hendriks M, van Luyn MJ
    J. Mol. Cell. Cardiol., 2006;41(1):86-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  56. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006;35(1):133-41.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  57. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006;120(2):220-31.
    Species: Human
    Sample Types: Blister Fluid
    Applications: Neut
  58. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
    Applications: ELISA Development
  59. Acidic extracellular pH promotes experimental metastasis of human melanoma cells in athymic nude mice.
    Authors: Rofstad EK, Mathiesen B, Kindem K, Galappathi K
    Cancer Res., 2006;66(13):6699-707.
    Species: Complex Species Category
    Sample Types:
    Applications: Neut
  60. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006;74(12):6730-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  61. Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells.
    Authors: Edwards MR, Johnson MW, Johnston SL
    Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  62. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006;119(10):2313-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  63. PAPP-A and osteoprotegerin, together with interleukin-8 and RANTES, are elevated in the peritoneal fluid of women with endometriosis.
    Authors: Bersinger NA, von Roten S, Wunder DM, Raio L, Dreher E, Mueller MD
    Am. J. Obstet. Gynecol., 2006;195(1):103-8.
    Species: Human
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  64. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
    Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
    Acta Obstet Gynecol Scand, 2005;84(6):551-7.
    Species: Human
    Sample Types: Amniotic Fluid
    Applications: ELISA Development
  65. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neut
  66. The activation of IL-8 receptors in cultured guinea pig Muller glial cells is modified by signals from retinal pigment epithelium.
    Authors: Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
    J. Neuroimmunol., 2005;161(1):49-60.
    Species: Guinea Pig
    Sample Types: Whole Cells
    Applications: ICC
  67. GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors.
    Authors: Penninckx F, Ectors N
    Exp. Cell Res., 2005;303(2):331-42.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  68. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005;46(6):659-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  69. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  70. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  71. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  72. ELR+ CXC chemokines in human milk.
    Authors: Maheshwari A, Christensen RD, Calhoun DA
    Cytokine, 2003;24(3):91-102.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  73. Interleukin-8 production in tuberculous pleurisy: role of mesothelial cells stimulated by cytokine network involving tumour necrosis factor-alpha and interleukin-1 beta.
    Authors: Park JS, Kim YS, Jee YK, Myong NH, Lee KY
    Scand. J. Immunol., 2003;57(5):463-9.
    Species: Human
    Sample Types: Pleural Smear
    Applications: ICC
  74. Selective induction of eotaxin release by interleukin-13 or interleukin-4 in human airway smooth muscle cells is synergistic with interleukin-1beta and is mediated by the interleukin-4 receptor alpha-chain.
    Authors: Hirst SJ, Hallsworth MP, Peng Q
    Am. J. Respir. Crit. Care Med., 2002;165(8):1161-71.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  75. Expression of functional CCR and CXCR chemokine receptors in podocytes.
    Authors: Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstadt H
    J. Immunol., 2002;168(12):6244-52.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  76. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  77. Metabolic replacement of kidney function in uremic animals with a bioartificial kidney containing human cells.
    Authors: Humes HD, Fissell WH, Weitzel WF, Buffington DA, Westover AJ, MacKay SM, Gutierrez JM
    Am. J. Kidney Dis., 2002;39(5):1078-87.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development
  78. Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation.
    Authors: Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA
    Immunity, 2001;14(6):705-14.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  79. Chemoattractant factors in breast milk from allergic and nonallergic mothers.
    Authors: Bottcher MF, Jenmalm MC, Bjorksten B, Garofalo RP
    Pediatr. Res., 2000;47(5):592-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  80. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
    Authors: Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
    Stem Cells, 0;34(4):1112-22.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  81. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 0;5(2):140-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut

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Human IL-8/CXCL8 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 11/01/2018
Application: MSD assay Sample Tested: Vitreous Humor Species: Cynomolgus Monkey,Porcine

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC).
A: Paired with SulfoTag-modified AF-208-NA as a detection antibody. Calibration curves with Recombinant Human IL-8 (208-IL-010/CF) is shown (dynamic range 1.6-25,000 pg/ml).
B: Paired with SulfoTag-modified MAB-535 to detect porcine IL-8. Calibration curves with Recombinant Porcine IL-8 (535-IN/CF) is shown (dynamic range 1.6-25,000 pg/ml).


Human IL-8/CXCL8 Antibody
By Anonymous on 05/11/2018
Application: B/N Sample Tested: Breast cancer cells Species: Human

We used this IL8 neutralizing antibody (600 ng/mL) as part of an antibody cocktail containing several other antibodies to neutralize IL8 present in the Conditioned Medium (CM) from BT549 Cells treated with Angiotensin II.
CM (+/- IL8 Neutralizing ab Cocktail) was used to further look at Endothelial (HUVEC) Cell migration using Incucyte Chemotaxis Assay.
Cancer Res; 78(5) March 1, 2018 (Fig 6F)


Human IL-8/CXCL8 Antibody
By Anonymous on 04/03/2018
Application: ELISA Sample Tested: Serum Species: Human

Human IL-8/CXCL8 Antibody
By Anonymous on 04/26/2017
Application: B/N Sample Tested: Neutrophils Species: Human