Human IL-9 R Antibody

(2 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-9 R in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL‑13 R alpha 1, rhIL-13 R alpha 2, or recombinant mouse IL-9 R is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 33423
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑9 R
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Neutralization
    Measured by its ability to neutralize IL‑9-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 2-4 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑9.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 R Antibody. Recombinant Human IL‑9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑9 R Subunit Mono­clonal Antibody (Catalog # MAB290). The ND50 is typically 2-4 µg/mL.
Detection of IL‑9 R in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human IL‑9 R Subunit Monoclonal Antibody (Catalog # MAB290, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-9 R
The specific high affinity functional IL-9 receptor complex contains the common gamma chain as well as the IL-9 receptor (IL-9 R). The mouse and human transmembrane proteins share 53% amino acid sequence identity. As a result of alternate splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.
  • Long Name:
    Interleukin 9 Receptor
  • Entrez Gene IDs:
    3581 (Human); 16199 (Mouse)
  • Alternate Names:
    CD129 antigen; CD129; IL-9 R; IL-9 receptor; IL9R; IL-9R; interleukin 9 receptor; interleukin-9 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Expression of IL-9 receptor alpha chain on human germinal center B cells modulates IgE secretion.
    Authors: Fawaz LM, Sharif-Askari E, Hajoui O, Soussi-Gounni A, Hamid Q, Mazer BD
    J. Allergy Clin. Immunol., 2007;120(5):1208-15.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Interleukin-9 induces goblet cell hyperplasia during repair of human airway epithelia.
    Authors: Vermeer PD, Harson R, Einwalter LA, Moninger T
    Am. J. Respir. Cell Mol. Biol., 2003;28(3):286-95.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 applications: Functional Assay, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 Functional Assay Human Anonymous 07/27/2016
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ApplicationFunctional Assay
Sample TestedPeripheral blood cells
SpeciesHuman
  Excellent
 Flow Human Anonymous 07/27/2016
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ApplicationFlow Cytometry
Sample TestedPeripheral blood cells
SpeciesHuman

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