Detects human IL-9 R in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-4 R, rhIL-5 R alpha, rhIL-5 R beta, rhIL‑13 R alpha 1, rhIL-13 R alpha 2, or recombinant mouse IL-9 R is observed.
Monoclonal Mouse IgG1 Clone # 33423
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑9 R
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
2.5 µg/106 cells
Measured by its ability to neutralize IL‑9-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 2-4 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑9.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 R Antibody. Recombinant Human IL‑9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑9 R Subunit Monoclonal Antibody (Catalog # MAB290). The ND50 is typically 2-4 µg/mL.
Detection of IL‑9 R in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human IL‑9 R Subunit Monoclonal Antibody (Catalog # MAB290, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-9 R
The specific high affinity functional IL-9 receptor complex contains the common gamma chain as well as the IL-9 receptor (IL-9 R). The mouse and human transmembrane proteins share 53% amino acid sequence identity. As a result of alternate splicing, cDNA clones encoding isoforms of IL-9 R, including a putative soluble form, have also been identified. Cells known to express IL-9 R include T cells, neutrophils, mast cells and macrophages.
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We have 2 reviews tested in 1
species: Human.We have 2 reviews tested in 2 applications: Functional Assay, Flow Cytometry.