Human Importin  alpha 2/KPNA2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Importin alpha 2/KPNA2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) KPNA1, rhKPNA3, rhKPNA4, rhKPNA5, or rhKPNB1 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 682208
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Importin alpha 2/KPNA2
    Ser2-Asn132
    Accession # P52292
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Importin alpha 2/KPNA2 in NTera‑2 Human Cell Line.

Importin alpha 2/KPNA2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cell line using Mouse Anti-Human Importin  alpha 2/KPNA2 Monoclonal Antibody (Catalog # MAB62071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Importin alpha 2/KPNA2
Importin subunit alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, and RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin b domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. The aminoacid region 1-132 of human Importin alpha 2 shares a 94% homology with mouse Importin alpha 2.
  • Long Name:
    Karyopherin alpha 2
  • Entrez Gene IDs:
    3838 (Human); 16647 (Mouse); 85245 (Rat)
  • Alternate Names:
    Importin alpha 1; Importin alpha 2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); Karyopherin subunit alpha-2; KPNA2; Pendulin; QIP2; QIP2importin alpha 2; RAG cohort 1; RAG cohort protein 1; RCH1; RCH1importin subunit alpha-2; SRP1; SRP1alpha; SRP1-alpha
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