Human Integrin  alpha 6/CD49f Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin  alpha 2, recombinant mouse (rm) Integrin  alpha 3, rhIntegrin  alpha 7, and rmIntegrin  alpha 7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Integrin alpha 6/CD49f
    Pro93-Arg148, Ile557-Arg613
    Accession # P23229
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Integrin  alpha 6/CD49f by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin  alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin  alpha 6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Human Integrin  alpha 6/CD49f by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue (cortex), loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha 6/CD49f at approximately 167 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Integrin  alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 6/CD49f

Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130‑150 kDa member of the integrin alpha chain family of molecules. Mature Integrin  alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24‑938), and a 30‑31 kDa membrane-bound light chain (aa 942‑1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa 215‑258 or a deletion of aa 260‑298, possibly accompanied by a 29 aa substitution for aa 1084‑1130. Over aa 93‑148 and 518‑574, human Integrin alpha 6 shares 89% aa identity with mouse Integrin alpha 6.

  • Entrez Gene IDs:
    3655 (Human); 16403 (Mouse)
  • Alternate Names:
    CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Beta4 integrin promotes osteosarcoma metastasis and interacts with ezrin.
    Authors: Wan X, Kim SY, Guenther LM, Mendoza A, Briggs J, Yeung C, Currier D, Zhang H, Mackall C, Li WJ, Tuan RS, Deyrup AT, Khanna C, Helman L
    Oncogene, 2009;28(38):3401-11.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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