Human Integrin alpha 6/CD49f Antibody

Catalog #: AF1350
2 Citations Datasheet / COA / SDS

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AF1350
AF1350-SP

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Detection of Human Integrin alpha 6/CD49f by Western Blot.

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin alpha 6/CD49f Antibody Summary

Species Reactivity

Human

Specificity

Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin alpha 2, recombinant mouse (rm) Integrin alpha 3, rhIntegrin alpha 7, and rmIntegrin alpha 7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Integrin alpha 6/CD49f
Pro93-Arg148, Ile557-Arg613
Accession # P23229

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Integrin alpha 6/CD49f by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin a6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western

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Detection of Human Integrin alpha 6/CD49f by Simple Western^TM. Simple Western lane view shows lysates of human kidney tissue (cortex), loaded at 0.2 mg/mL. A specific band was detected for Integrin a6/CD49f at approximately 167 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Integrin a6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 6/CD49f

Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130‑150 kDa member of the integrin alpha chain family of molecules. Mature Integrin alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24‑938), and a 30‑31 kDa membrane-bound light chain (aa 942‑1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa 215‑258 or a deletion of aa 260‑298, possibly accompanied by a 29 aa substitution for aa 1084‑1130. Over aa 93‑148 and 518‑574, human Integrin alpha 6 shares 89% aa identity with mouse Integrin alpha 6.

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Alternate Names

CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Integrin alpha 6/CD49f Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Identification of distinct N-glycosylation patterns on extracellular vesicles from small-cell and non-small-cell lung cancer cells
Authors: K Kondo, Y Harada, M Nakano, T Suzuki, T Fukushige, K Hanzawa, H Yagi, K Takagi, K Mizuno, Y Miyamoto, N Taniguchi, K Kato, T Kanekura, N Dohmae, K Machida, I Maruyama, H Inoue
The Journal of Biological Chemistry, 2022-04-18;0(0):101950.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Beta4 integrin promotes osteosarcoma metastasis and interacts with ezrin.
Authors: Wan X, Kim SY, Guenther LM, Mendoza A, Briggs J, Yeung C, Currier D, Zhang H, Mackall C, Li WJ, Tuan RS, Deyrup AT, Khanna C, Helman L
Oncogene, 2009-07-13;28(38):3401-11.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot