Human Integrin alpha 6/CD49f Antibody

R&D Systems | Catalog # AF1350

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin alpha 6/CD49f Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Integrin alpha 6/CD49f
Pro93-Arg148, Ile557-Arg613
Accession # P23229

Specificity

Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin  alpha 2, recombinant mouse (rm) Integrin  alpha 3, rhIntegrin  alpha 7, and rmIntegrin  alpha 7 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Integrin alpha 6/CD49f Antibody

Detection of Human Integrin a6/CD49f antibody by Western Blot.

Detection of Human Integrin alpha 6/CD49f by Western Blot.

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin a6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Integrin a6/CD49f antibody by Simple WesternTM.

Detection of Human Integrin alpha 6/CD49f by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (PC‑3) (NBP2-49856) and human kidney tissue, loaded at 0.5 mg/ml. A specific band was detected for Integrin  alpha 6/CD49f at approximately 163 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Integrin  alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Applications for Human Integrin alpha 6/CD49f Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Exosome Standard (PC-3) (Catalog # NBP2-49856) and human kidney tissue

Western Blot

1 µg/mL
Sample: Human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 6/CD49f

Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130‑150 kDa member of the integrin alpha chain family of molecules. Mature Integrin  alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24‑938), and a 30‑31 kDa membrane-bound light chain (aa 942‑1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa 215‑258 or a deletion of aa 260‑298, possibly accompanied by a 29 aa substitution for aa 1084‑1130. Over aa 93‑148 and 518‑574, human Integrin alpha 6 shares 89% aa identity with mouse Integrin alpha 6.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Gene Symbol

ITGA6

UniProt

P23229

Additional Integrin alpha 6/CD49f Products

Product Documents for Human Integrin alpha 6/CD49f Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Integrin alpha 6/CD49f Antibody

For research use only

Citations for Human Integrin alpha 6/CD49f Antibody

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