Detects human Integrin alpha 6/CD49f in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Integrin alpha 9 is observed and less than 1% cross-reactivity with rhIntegrin alpha 2, recombinant mouse (rm) Integrin alpha 3, rhIntegrin alpha 7, and rmIntegrin alpha 7 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human Integrin alpha 6/CD49f Pro93-Arg148, Ile557-Arg613 Accession # P23229
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Integrin alpha 6/CD49f by Western Blot.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 ug/mL of Goat Anti-Human Integrin alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin alpha 6/CD49f at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Integrin alpha 6/CD49f by Simple WesternTM.
Simple Western lane view shows lysates of human kidney tissue (cortex), loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 6/CD49f at approximately 167 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Integrin alpha 6/CD49f Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1350) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 6/CD49f
Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130‑150 kDa member of the integrin alpha chain family of molecules. Mature Integrin alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24‑938), and a 30‑31 kDa membrane-bound light chain (aa 942‑1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin. Splice variants exist that show either a deletion of aa 215‑258 or a deletion of aa 260‑298, possibly accompanied by a 29 aa substitution for aa 1084‑1130. Over aa 93‑148 and 518‑574, human Integrin alpha 6 shares 89% aa identity with mouse Integrin alpha 6.
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The data collected includes not only links to publications in PubMed,
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