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Human Integrin  alpha L/CD11a Antibody

Catalog #: MAB3595
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3595
MAB3595-SP

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Detection of Human Integrin alpha L/CD11a by Western Blot.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  alpha L/CD11a Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha L in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 345913
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Integrin  alpha L
Tyr26-Met1089 (Tyr660Ile)
Accession # CAA68747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
PBMC, Placenta

Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin aL/CD11a antibody by Western Blot. View Larger

Detection of Human Integrin alpha L/CD11a by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Human Integrin aL/CD11a Monoclonal Antibody (Catalog # MAB3595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin aL/CD11a at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western View Larger

Detection of Human Integrin  alpha L/CD11a by Simple WesternTM. Simple Western lane view shows lysates of PBMC, Placenta, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha L/CD11a at approximately 220 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Integrin  alpha L/CD11a Monoclonal Antibody (Catalog # MAB3595). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha L/CD11a

Integrin subunit alpha L/CD11a is a 180 kDa type I TM glycoprotein that interacts only with Integrin  beta 2/CD18 to form LFA-1, a leukocyte adhesion protein which binds endothelial cell ICAM. Human Integrin alpha L contains a 1064 aa extracellular domain (ECD), a 20 aa TM sequence and a 58 aa cytoplasmic domain. The ECD contains seven repeats that form a beta-propeller structure and one inserted vWA domain (I domain) containing a metal ion-dependent adhesion site (MIDAS). Human and mouse Integrin alpha L ECD share 74% aa identity. A second isoform has a 53 aa insert in the ECD.

Entrez Gene IDs
3683 (Human); 16408 (Mouse); 308995 (Rat)
Alternate Names
CD11 antigen-like family member A; CD11a antigen; CD11a; CD11Aantigen CD11A (p180), lymphocyte function-associated antigen 1, alphapolypeptide; Integrin alpha L; integrin alpha-L; integrin gene promoter; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen1; alpha polypeptide); ITGAL; Leukocyte adhesion glycoprotein LFA-1 alpha chain; Leukocyte function-associated molecule 1 alpha chain; LFA-1 alpha; LFA-1; LFA1A; LFA-1A; Ly-15; Ly-21; lymphocyte function-associated antigen 1

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Citations for Human Integrin  alpha L/CD11a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Aggregatibacter actinomycetemcomitans Leukotoxin (LtxA) Requires Death Receptor Fas, in Addition to LFA-1, To Trigger Cell Death in T Lymphocytes
    Authors: Brian A. Vega, Liam T. Schober, Tami Kim, Benjamin A. Belinka, Scott C. Kachlany
    Infection and Immunity
  2. The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
    Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
    Arthritis Rheum., 2007-06-01;56(6):1817-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007-05-01;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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