|Detection of Human IRF2BP1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and MOLT‑4 human acute lymphoblastic leukemia cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF‑ beta 1 (Catalog # 240-B) for 2 hours. Gels were loaded with 30 µg of whole cell lysate (WCL) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF2BP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
|IRF2BP1 in 293T Human Cell Line. IRF2BP1 was detected in immersion fixed 293T human embryonic kidney cell line using Sheep Anti-Human IRF2BP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6800) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.|
IRF2BP1 (Interferon regulatory factor 2 binding protein 1) is a 64-66 kDa member of the IFR2BP family of proteins. It is widely expressed, and known to bind to the C-terminus of IRF-2, creating a transcriptional repressor complex. IRF2BP1 also bind to JDP2 (Jun dimerization protein 2), promoting its ubiquitination. Human IRF2BP1 is 584 amino acids (aa) in length. It contains two Zn-finger domains (aa 6-67 and 498-584), one coiled-coil region (aa 197-217), a Cys-rich region (aa 503‑550), an N-terminal acetylation site at Ala2, and multiple utilized phosphorylation sites at Ser 66/125/186/384/421/436/453/457. Over aa 188-356, human and mouse IRF2BP1 are identical in amino acid sequence.
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