Human Klotho DuoSet ELISA

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Ancillary Products Available
Human Klotho ELISA Standard Curve
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Product Details
Citations (10)
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Reviews (1)

Human Klotho DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Klotho. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human Klotho ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Klotho

The Klotho proteins, Klotho and Klotho beta (beta KL), are type I transmembrane glycoproteins with extracellular regions that contain two beta-glycosidase-like repeats. Klotho has both membrane-bound and soluble forms; the soluble form predominates and consists of only the first beta-glycosidase-like repeat. Klotho has beta-glucuronidase activity against select steroid beta-glucuronides, appears to regulate calcium and vitamin D levels in serum, and is associated with premature aging.

Klotho beta, a divergent structural member of the glycosidase I superfamily, is primarily expressed in the liver and pancreas, with lower expression in adipose tissue. Klotho beta mediates the interaction of FGF-15 (human FGF-19) with FGF Receptor 4 in the liver, which negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes. Klotho beta is also a cofactor for the interaction of FGF-21 with FGF Receptor 1c in adipocytes. The expression pattern of Klotho beta suggests it may play a role in lipid metabolism.

Entrez Gene IDs:
9365 (Human); 16591 (Mouse)
Alternate Names:
EC 3.2.1; EC; KL; Klotho

Citations for Human Klotho DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Effects of low-dose ketamine infusion on vascular endothelial growth factor and matrix metalloproteinase-9 among patients with treatment-resistant depression and suicidal ideation
    Authors: Chen, MH;Lin, WC;Li, CT;Wu, HJ;Bai, YM;Tsai, SJ;Su, TP;Tu, PC;
    Journal of psychiatric research
    Species: Human
    Sample Types: Serum
  2. The Circulating Level of Klotho Is Not Dependent upon Physical Fitness and Age-Associated Methylation Increases at the Promoter Region of the Klotho Gene
    Authors: D Aczel, F Torma, M Jokai, K McGreevy, A Boros, Y Seki, I Boldogh, S Horvath, Z Radak
    Genes, 2023-02-19;14(2):.
    Species: Human
    Sample Types: Plasma
  3. Association of serum klotho levels with different-staged vascular calcification status in patients with maintenance hemodialysis
    Authors: Y Lin, J Huang, M Zhou, C Pan, A Shan, C Zhou
    Bmc Nephrology, 2022-11-19;23(1):374.
    Species: Human
    Sample Types: Serum
  4. Combined resistance and aerobic training improves lung function and mechanics and fibrotic biomarkers in overweight and obese women
    Authors: A Silva-Reis, MA Rodrigues, R Moraes-Fer, TG Gonçalves-, VH Souza-Palm, HC Aquino-San, ALL Bachi, LVF de Oliveir, RÁB Lopes-Mart, I Oliveira-S, R Albertini, CR Frison, RP Vieira
    Frontiers in Physiology, 2022-09-07;13(0):946402.
    Species: Human
    Sample Types: Plasma
  5. Klotho and SIRT1 changes from pre-diabetes to diabetes and pre-hypertension to hypertension
    Authors: M Yeganeh-Ha, H Najafipour, F Rostamzade, A Naghibzade
    Diabetology & metabolic syndrome, 2021-10-20;13(1):115.
    Species: Human
    Sample Types: Serum
  6. Pulmonary function changes in older adults with and without metabolic syndrome
    Authors: MAR Brandao-Ra, R Moraes-Fer, MC Oliveira-J, A Santos-Dia, ALL Bachi, G Gabriela-P, S de Oliveir, AC Araújo-Ros, LVF Oliveira, CR Frison, WL do Prado, RP Raju, PB Balagopal, RP Vieira
    Scientific Reports, 2021-08-30;11(1):17337.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)
  7. No significant association of serum klotho concentration with blood pressure and pulse wave velocity in a Chinese population
    Authors: WY Liang, LH Wang, JH Wei, QL Li, QY Li, Q Liang, NQ Hu, LH Li
    Scientific Reports, 2021-01-27;11(1):2374.
    Species: Human
    Sample Types: Serum
  8. Circulating Levels of Soluble Klotho and Fibroblast Growth Factor 23 in Mexican Diabetic Patients and Its Association With Early Nephropathy
    Authors: A Farías-Bas, HR Martínez-R, EF Gómez-Garc, AM Cueto-Manz, L Cortés-San, LE Hernández-, G Ramírez-Ló, F Mendoza-Ca
    Arch. Med. Res., 2019-02-01;0(0):.
    Species: Human
    Sample Types: Serum
  9. Associations of sclerostin with carotid artery atherosclerosis and all-cause mortality in Chinese patients undergoing maintenance hemodialysis
    Authors: A Chen, Y Sun, J Cui, B Zhao, H Wang, X Chen, Y Mao
    BMC Nephrol, 2018-10-12;19(1):264.
    Species: Human
    Sample Types: Plasma
  10. Sclerostin, TNF-alpha and Interleukin-18 Correlate and are Together with Klotho Related to Other Growth Factors and Cytokines in Haemodialysis Patients.
    Authors: Almroth G, Lonn J, Uhlin F, Brudin L, Andersson B, Hahn-Zoric M
    Scand J Immunol, 2016-01-01;83(1):58-63.
    Species: Human
    Sample Types: Serum


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Human Klotho DuoSet ELISA
By Anonymous on 11/22/2017
Sample Tested: HEK293 human embryonic kidney cell line,Human Kidney cell line HK-2

The ELISA clearly recognizes Klotho Full Length in transfected and non-transfected human kidney cell lines