Detection of Human Kynureninase by Western Blot. Western blot shows lysates of human liver tissue and A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Kynureninase Monoclonal Antibody (Catalog # MAB4887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Kynureninase at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Kynureninase (KYNU) catalyzes the hydrolytic cleavage of the amino acids L-kynurenine and L-3-hydroxykynurenine to give either anthranilic acid or 3‑hydroxyanthranilic acid and alanine. KYNU and other “kynurenine pathway” enzymes degrade dietary tryptophan in the liver and are involved in the de novo biosynthesis of NAD+. KYNU and other pathway proteins in immune system cells, such as macrophages and microglia, catalyze inflammatory quinolinic acid (QA) production, which may cause neuronal damage in AIDS-related dementia complex, Alzheimer’s, stroke, epilepsy, and Huntington’s disease. Human KYNU shares 83% and 85% amino acid identity with mouse and human KYNU, respectively.
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