Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 84321
View all L1CAM Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human L1CAM
Met1-Glu1120
Accession # CAA42508

Specificity

Detects human L1CAM in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) VCAM-1, rhICAM-1, rhICAM-2, and rhICAM-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human L1CAM Antibody

Detection of Human L1CAM by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human brain (motor cortex). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human L1CAM Monoclonal Antibody (Catalog # MAB777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for L1CAM at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human L1CAM by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for L1CAM at approximately 337 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human L1CAM Monoclonal Antibody (Catalog # MAB777). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
Detection of Mouse L1CAM by Immunohistochemistry

Detection of Mouse L1CAM by Immunohistochemistry

Analysis of L1 and pCK2 alpha immunofluorescence intensities rostral, central and caudal to the lesion site of the injured spinal cord at 6 weeks post-SCI in mice treated with trimebutine or honokiol. (A,C) Representative images of L1 (A) and pCK2 alpha (C) rostral (1 mm to the lesion center), central and caudal (1 mm to the lesion center) to the lesion site. (B,D) Quantitative analysis of L1 (B) and pCK2 alpha (D) immunoreactivities. *P<0.05, **P<0.01 versus the vehicle control; one-way ANOVA with Tukey's post hoc test; n=3 mice/group. HNK, honokiol; TMB, trimebutine; VC, vehicle control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714852), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse L1CAM by Western Blot

Detection of Mouse L1CAM by Western Blot

Activation of L1-mediated intracellular signaling cascades in cultured mouse cerebellar granule cells treated with trimebutine. (A-E) Protein levels of L1 (A), phosphorylation levels of Akt1 (B) and Erk1/2 (C), protein levels of mTOR (D), and the ratio of Bcl-2/Bax levels (E) in cultured mouse cerebellar granule cells treated with trimebutine (TMB) at concentrations of 0, 5, 10 and 20 nM. *P<0.05, **P<0.01, ***P<0.001 versus the vehicle control; one-way ANOVA with Tukey's post hoc test; three independent experiments. Blots in C-E are from the same gel and therefore have the same beta -actin loading controls. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714852), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse L1CAM by Western Blot

Detection of Mouse L1CAM by Western Blot

Activation of L1-mediated intracellular signaling cascades at 6 weeks after SCI in mice treated with trimebutine or honokiol. Western blot analysis of L1 (A), phosphorylation levels of Akt1 (B), Erk1/2 (C), CK2 alpha (D) and mTOR (E), protein levels of PTEN (F), p53 (G) and GFAP (H), and Bcl-2/Bax ratio (I) of mice treated with vehicle control (VC), trimebutine (TMB) or honokiol (HNK) at 5 mm rostral to the lesion center, the lesion center, and 5 mm caudal to the lesion center. *P<0.05, **P<0.01 versus the vehicle control; one-way ANOVA with Tukey's post hoc test; n=4 mice/group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28714852), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human L1CAM Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and human brain (motor cortex)

Reviewed Applications

Read 1 review rated 5 using MAB777 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: L1CAM

L1CAM (Neural cell adhesion molecule L1, also known as L1, CD171 and NCAM-L1) is a 200-230 kDa member of the L1 family, Immunoglobulin (Ig) superfamily of molecules. L1 is recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation. It does so through a series of cis and trans interactions that involve multiple copartners and target receptors. L1 is described as forming both homotypic and heterotypic complexes, the latter with molecules as diverse as the EGFR, NCAM, CD24, neurocan and various alpha v plus beta 1 and beta 3 integrins. Cells known to express L1 include immature oligodendrocytes, CD4+ T cells, B cells and monocytes, premyelinating Schwann cells, intestinal epithelial progenitor cells, and cerebellar granule plus Purkinje cells. Mature human L1 is a 1238 amino acid (aa) type I transmembrane protein. It contains an 1101 aa extracellular region (aa 20-1120) plus a 114 aa cytoplasmic domain (aa 1144-1257). The extracellular region possesses six C2-type Ig-like domains (aa 35-607) followed by five fibronectin (FN) type III repeats (aa 612-1108). The cytoplasmic tail contains no kinase motifs, but does possess a FIGQY peptide that interacts with ankyrin, and an RSLE sequence that mediates clathrin-associated endocytosis. At least five Ser residues are known to be phosphorylated. There are two splice variants, one each in the intracellular and extracellular domain. A deletion of RSLE (aa 1177-1180) adversely affects endocytosis, while a Leu substitution for aa 26-31 interfers with numerous heterotypic interactions. In general, the full-length L1 molecule is a neuron-associated isoform. L1 is known to undergo proteolysis, either by plasmin or ADAMs. This generates soluble isoforms of varying sizes (140-200 kDa) that retain bioactivity, and which can be incorporated into the surrounding ECM. The membrane fragments (30-80 kDa) undergo further processing, most importantly by gamma -secretase, to generate a soluble 28 kDa intracellular domain. This domain is SUMOylated, and believed to possess an NLS at Lys1147. Upon presumed entry into the nucleus, L1 is posited to activate L1-responsive genes. In the extracellular region, human and mouse L1 share 86% aa sequence identity.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Entrez Gene IDs

3897 (Human); 16728 (Mouse); 50687 (Rat)

Gene Symbol

L1CAM

UniProt

CAA42508

Additional L1CAM Products

Product Documents for Human L1CAM Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human L1CAM Antibody

For research use only

Citations for Human L1CAM Antibody

Customer Reviews for Human L1CAM Antibody (1)

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  • Human L1CAM Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/17/2019
    MAB777 combined with AF277 in a LBA to measure L1CAM in human serum samples.
    Human L1CAM Antibody MAB777

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