Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 586439
View all LILRA1/CD85i/LIR-6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human LILRA1/CD85i/LIR-6
Pro17-Asn461
Accession # O75019

Specificity

Detects human LILRA1/CD85i/LIR-6 in direct ELISAs. In direct ELISAs, no cross-reactivity with human LILRA2, LILRA3, LILRA4, LILRA5, LILRA6, LILRB2 and LILRB3 was observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LILRA1/CD85i/LIR-6 Antibody

LILRA1/CD85i/LIR-6 antibody in THP-1 Human Cell Line and PBMCs by Immunocytochemistry (ICC).

LILRA1/CD85i/LIR-6 in THP-1 Human Cell Line and PBMCs.

LILRA1/CD85i/LIR-6 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel, positive stain) and human peripheral blood mononuclear cells (PBMCs; right panel, negative stain) using Mouse Anti-Human LILRA1/CD85i/LIR-6 Monoclonal Antibody (Catalog # MAB30852) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human LILRA1/CD85i/LIR-6 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed THP-1 human acute monocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRA1/CD85i/LIR-6

Leukocyte Immunoglobulin-like Receptor 6 (LIR-6), also called LILRA1 or CD85i, is a glycoprotein member of the LIR family of leukocyte Ig-like receptors. As an activating LIR, LIR-6 has a short cytoplasmic tail and a charged aa within the TM domain which interacts with FcR gamma. Both four (LIR-6a) and two (LIR-6b) Ig-like domain forms are expressed by monocytes and B cells. Human LIR-6 has no non-primate ortholog, but shows up to 80% aa identity with human ILT-1c and KIR-p91/PIR-B. A mouse homolog, gp49B2, has structural similarity but limited sequence identity.

Long Name

Leukocyte Immunoglobulin-Like Receptor, Subfamily A (With TM Domain), Member 1

Alternate Names

CD85i, LIR-6, LIR6

Entrez Gene IDs

11024 (Human)

Gene Symbol

LILRA1

UniProt

O75019

Additional LILRA1/CD85i/LIR-6 Products

Product Documents for Human LILRA1/CD85i/LIR-6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LILRA1/CD85i/LIR-6 Antibody

For research use only

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