Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1057017
View all LILRB3/CD85a/ILT5 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human LILRB3/CD85a/ILT5
Gly24-Glu443
Accession # O75022

Specificity

Detects human LILRB3/CD85a/ILT5 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human LILRB3/CD85a/ILT5 Antibody

Detection of LILRB3/CD85a/ILT5 in human neutrophils with CD15 costain cells by Flow Cytometry.

Human neutrophils with CD15 costain cells were stained with Mouse Anti-Human/Mouse SSEA‑1 PE‑conjugated Monoclonal Antibody (Catalog # FAB2155P) and either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB18061) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of LILRB3/CD85a/ILT5 in PBMC with CD14 costain cells by Flow Cytometry.

PBMC with CD14 costain were stained with either (A) Mouse Anti-Human LILRB3/CD85a/ILT5 Monoclonal Antibody (Catalog # MAB18061) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human LILRB3/CD85a/ILT5 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Human neutrophils with CD15 costain and PBMCs with CD14 costain

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LILRB3/CD85a/ILT5

Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1. Subfamily A members are activating receptors that lack ITIMs and signal through association with FcR gamma (1, 2). Mature LILRB3 is a highly polymorphic 85‑95 kDa glycoprotein that consists of a 420 amino acid (aa) extracellular domain (ECD) with four Ig-like domains, a 21 aa transmembrane segment, and a 167 aa cytoplasmic domain with three ITIMs (3). Alternative splicing generates an isoform with a 17 aa insertion in the juxtamembrane ECD. In mouse and rat, the LILRB3 gene encodes the PIR-B protein which has six Ig-like domains. Rodent PIR-B and human LILRB3 share 55% aa sequence identity within common regions of their ECDs. LILRB3 is expressed on the surface of peripheral monocytes, neutrophils, eosinophils, basophils, and mast cell progenitors (4-6). Triggering of LILRB3 inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5, 7). On osteoclast precursors, LILRB3 ligation inhibits RANK L/TRANCE or M-CSF induced differentiation (8). LILRB3 can also bind to ligands exposed on necrotic tumor cells (9). Both PIR-B and LILRB3 are receptors for S. aureus, and activation of these receptors by bacteria influences the innate immune response triggered by TLRs (3). R&D Systems in-house testing indicates that LILRB3 binds to
Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (10). In the mouse CNS, PIR-B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (11). Upon binding to MAG, PIR-B associates with TrkB and NGF R/p75 in cerebellar granule neurons (12).

References

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
  3. Nakayama, M. et al. (2007) J. Immunol. 178:4250.
  4. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1174.
  5. Sloane, D.E. et al. (2004) Blood 104:2832.
  6. Tedla, N. et al. (2008) J. Leukoc. Biol. 83:334.
  7. Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
  8. Mori, Y. et al. (2008) J. Immunol. 181:4742.
  9. Jones, D.C. et al. (2016) Oncotarget PMID 26769854.
  10. Zheng, J. et al. (2012) Nature 485:656.
  11. Atwal, J.K. et al. (2008) Science 322:967.
  12. Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.

Alternate Names

CD85a, ILT5, LIR3

Entrez Gene IDs

11025 (Human)

Gene Symbol

LILRB3

UniProt

O75022 (Human)

Additional LILRB3/CD85a/ILT5 Products

Product Documents for Human LILRB3/CD85a/ILT5 Antibody

Certificate of Analysis

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Product Specific Notices for Human LILRB3/CD85a/ILT5 Antibody

For research use only

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