Livin alpha (KIAP) and Livin beta (ML-IAP) are two splice forms generated from the livin gene. Livin is a member of the inhibitor of apoptosis protein (IAP) family that contains a baculovirus single IAP repeat (BIR) and a C-terminal RING finger domain. Livin inhibits the activation of caspases 9 and/or 3 when activated in specific cell lines. Livin alpha is a splice variant 18 amino acids longer in the BIR-RING linking region than the livin beta splice variant. The isoforms differ in their apoptotic properties.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Livin beta
Met1-Gln280
Accession # Q96CA5
Met1-Gln280
Accession # Q96CA5
Specificity
Detects human Livin in Western blots. Can detect recombinant and endogenous human Livin alpha (KIAP) and Livin beta (ML-IAP).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Livin Antibody
Detection of Human Livin by Western Blot.
Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, Recombinant Human Livin a/KIAP (Catalog # 1161-LV) and Recombinant Human Livin beta /ML-IAP (Catalog # 787-LV) (0.5 ng/lane) were included. A specific band for Livin was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human Livin by Simple WesternTM.
Simple Western lane view shows lysates of SK-Mel-28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Livin at approximately 42 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Applications for Human Livin Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: SK‑Mel‑28 human malignant melanoma cell line
Sample: SK‑Mel‑28 human malignant melanoma cell line
Western Blot
0.5 µg/mL
Sample: SK-Mel-28 human malignant melanoma cell line
Sample: SK-Mel-28 human malignant melanoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Livin
Additional Livin Products
Product Documents for Human Livin Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Livin Antibody
For research use only
Related Research Areas
Citations for Human Livin Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
