Human Livin Antibody Summary
Accession # Q96CA5
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Livin by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, Recombinant Human Livin a/KIAP (Catalog # 1161-LV) and Recombinant Human Livin beta /ML-IAP (Catalog # 787-LV) (0.5 ng/lane) were included. A specific band for Livin was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human Livin by Simple WesternTM. Simple Western lane view shows lysates of SK-Mel-28 human malignant melanoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Livin at approximately 42 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Livin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1161) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Livin alpha (KIAP) and Livin beta (ML-IAP) are two splice forms generated from the livin gene. Livin is a member of the inhibitor of apoptosis protein (IAP) family that contains a baculovirus single IAP repeat (BIR) and a C-terminal RING finger domain. Livin inhibits the activation of caspases 9 and/or 3 when activated in specific cell lines. Livin alpha is a splice variant 18 amino acids longer in the BIR-RING linking region than the livin beta splice variant. The isoforms differ in their apoptotic properties.
Citations for Human Livin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Dacarbazine and the agonistic TRAIL receptor-2 antibody lexatumumab induce synergistic anticancer effects in melanoma.
Authors: Engesaeter B, Engebraaten O, Florenes V, Maelandsmo G
PLoS ONE, 2012;7(9):e45492.
Sample Types: Cell Lysates
Applications: Western Blot
Serum levels of inhibitors of apoptotic proteins (IAPs) change with IVIg therapy in pemphigus.
Authors: Toosi, Siavash, Habib, Nancy, Torres, Geneviev, Reynolds, Sandra R, Bystryn, Jean-Cla
J Invest Dermatol, 2011;131(11):2327-9.
Sample Types: Serum
Applications: Western Blot
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