Human LRH-1/NR5A2 Antibody

Catalog #: PP-H2325-00
8 Citations Datasheet / COA / SDS

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Citations (8)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human LRH-1/NR5A2 Antibody Summary

Species Reactivity

Human

Specificity

This antibody specifically recognizes human LRH-1 alpha and LRH-1 beta and cross-reacts with mouse and rat LRH-1. Not yet tested in other species.

Source

Monoclonal Mouse IgG₃ Clone # H2325

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant human LRH-1/NR5A2
aa 161-280

Formulation

A liquid formulation in physiologic saline with 0.1% NaN₃.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

This antibody can be used at 1 μg/mL with the appropriatesecondary reagents to detect human LRH-1.

Immunoprecipitation

Optimal dilutions should be determined by eachlaboratory.

Direct ELISA

This antibody can be used at 2 ng/mL with the appropriatesecondary reagents to detect human LRH-1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody is stable for greater than six months when held at -20 °C in a manual defrost freezer or at -70 °C. Upon thawing, the antibody can be stored at 2-8 °C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Background: LRH-1/NR5A2

Liver Receptor Homologous Protein (LRH-1, FTF, CPF, hB1F, FTZ-F1b; NR5A2) is a member of the Orphan Nuclear Receptor family. LRH-1 is a mammalian homolog of the Drosophila Fushi Tarazu F1 Gene product. LRH-1 is expressed in the liver and intestine. LRH-1 is involved in the regulation of genes which participate in steroid, bile acid and cholesterol homeostasis.

Long Name

Liver Receptor Homolog 1/Nuclear Receptor Subfamily 5, Group A, Member 2

Entrez Gene IDs

2494 (Human)

Alternate Names

Alpha-1-fetoprotein transcription factor; B1-binding factor; b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus; B1F; B1F2; fetoprotein-alpha 1 (AFP) transcription factor; FTFLRH1; FTZ-F1 beta; FTZ-F1; FTZ-F1beta; HB1F; hB1F-2; hB1FCYP7A promoter-binding factor; Hepatocytic transcription factor; Liver receptor homolog 1; liver receptor homolog-1; LRH1; LRH-1; LRH-1CPF; NR5A2; nuclear receptor NR5A2; nuclear receptor subfamily 5 group A member 2; nuclear receptor subfamily 5, group A, member 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Steroid Hormone Receptors and Regulators

Citations for Human LRH-1/NR5A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Reprogramming competence of OCT factors is determined by transactivation domains
Authors: KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler
Science Advances, 2020;6(36):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Steroidogenic control of liver metabolism through a nuclear receptor-network
Authors: A Milona, V Massafra, H Vos, J Naik, N Artigas, HAB Paterson, ITGW Bijsmans, ECL Willemsen, JM Ramos Pitt, I Miguel-Ali, P Bosma, BMT Burgering, C Williamson, S Vernia, WS Dhillo, SWC van Mil, BM Owen
Mol Metab, 2019;30(0):221-229.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ChIP
LRH-1 regulates hepatic lipid homeostasis and maintains arachidonoyl phospholipid pools critical for phospholipid diversity
Authors: DA Miranda, WC Krause, A Cazenave-G, M Suzawa, H Escusa, JC Foo, DS Shihadih, A Stahl, M Fitch, E Nyangau, M Hellerstei, MR Wenk, DL Silver, HA Ingraham
JCI Insight, 2018;3(5):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis
Authors: A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang
J. Biol. Chem., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
LRH-1 mitigates intestinal inflammatory disease by maintaining epithelial homeostasis and cell survival
Authors: JR Bayrer, H Wang, R Nattiv, M Suzawa, HS Escusa, RJ Fletterick, OD Klein, DD Moore, HA Ingraham
Nat Commun, 2018;9(1):4055.
Species: Human
Sample Types: Organoids
Applications: Western Blot
Silencing LRH-1 in colon cancer cell lines impairs proliferation and alters gene expression programs.
Authors: Bayrer J, Mukkamala S, Sablin E, Webb P, Fletterick R
Proc Natl Acad Sci U S A, 2015;112(8):2467-72.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
LRH-1 and PTF1-L coregulate an exocrine pancreas-specific transcriptional network for digestive function.
Authors: Holmstrom SR, Deering T, Swift GH
Genes Dev., 2011;25(16):1674-9.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ChIP
A genome-wide screen in EpiSCs identifies Nr5a nuclear receptors as potent inducers of ground state pluripotency.
Authors: Guo G, Smith A
Development, 2010;137(19):3185-92.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot